GA_3、6-BA对金钗石斛生长发育及生物碱含量的影响

Influence of exogenous GA_3 and 6-BA on the growth and alkaloid content in Dendrobium nobile

在金钗石斛(DendrobiumnobileLindl.)高分蘖期前用GA3和6 BA进行浸根处理,分析GA3和6 BA对金钗石斛生长发育及生物碱含量的影响。结果表明:GA3、6 BA处理使金钗石斛SOD、POD活性升高,可溶性蛋白质和可溶性糖含量增加;用0.5～1.0mg·L-1GA3和10mg·L-16 BA处理可使金钗石斛的总生物碱含量分别增加6.7%～10.1%和3.4%;石斛碱的含量不受GA3和6 BA的影响。0.5～1.0mg·L-1GA3和10～50mg·L-16 BA处理可使金钗石斛生物产量分别增加13.3%～30.7%和17.3%～40.0%,有效分蘖率提高63.2%～68.9%和63.1%～82.1%,GA3可显著促进茎的延长生长。内源IAA含量在5-8月份较高,其中以6月份含量最高(4.84μg·g-1),1月份最低(2.97μg·g-1);内源ABA含量在1月份达最高(0.61μg·g-1)。用含有1.0mg·L-1GA3和50mg·L-16 BA的激素营养液处理可以显著提高金钗石斛生物产量(比CK增产38%),而且总生物碱含量也增加5.6%。

In order to research the influence of GA_(3) and 6-BA on the growth of Dendrobium nobile Lindl., the roots were soaked in different concentrations of GA_(3) and 6-BA before the peak tillering stage. The results showed that treatments with GA_(3) and 6-BA could increase the activities of SOD and POD as well as the contents of soluable protein and soluable sugar. Treatments with 0.5-1.0 mg·L^(-1) GA_(3) and 10 mg·L^(-1) 6-BA could increase the contents of total alkaloid by 6.7%-10.1% and 3.4% respectively. Dendrobine content was not susceptible to GA_(3) or 6-BA. Results of output analysis showed that treatments with 0.5-1.0 mg·L^(-1) GA_(3) and 10-50 mg·L^(-1) 6-BA could increase the biological output by 13.3%-30.7% and 17.3%-40.0% respectively, the effective tillering rate by 63.2%-68.9% and 63.1%-82.1% respectively and GA_(3) could markedly enhance the lenthening growth of the stem. The content of endohormone IAA was generally high during May to August and reached the climax in June (4.84μg·g^(-1)),the bottom in January (2.97μg·g^(-1)); endohormone ABA reached the climax in January (0.61 μg·g^(-1)). The nutrient solution with 1.0 mg·L^(-1) GA_(3) and 50 mg·L^(-1) 6-BA obviously increased biological output of D. nobile (increased by 38% compared with CK) and the total alkaloid content was increased by 5.6%.