小檗碱-I_3^-缔合微粒体系的光度分析应用

Spectrophotometric Analytical Application of the Berberine-I_3^- Association Particle System

在稀HCl介质中,I_3^-在340 nm处有一吸收峰;当小檗碱(BB)与I_3^-共存时体系呈橙黄色,在580 nm处产生一共振散射峰。以试剂作参比,该缔舍微粒体系在530 nm产生一吸收峰,BB浓度在0～7.0×10^(-5)mol/L范围内与A_(530nm)呈线性,据此建立了一种测定小檗碱含量的分光光度新方法,并用于针剂样品中小檗碱测定,结果满意。同步散射光谱研究表明,BB^+与I_3^-可通过静电引力作用形成疏水性的(I_3-BB)缔合物分子,并进一步聚集形成稳定的(I_3-BB)_n缔合纳米微粒。由于该缔合纳米微粒在580 nm处产生共振散射效应,故体系呈橙黄色。

In dilute HCl medium, there is a maximum absorption peak at 340 nm for I_3^-. When berberine (BB) and I_3^- are co-existent, it is in orange-yellow color, and exhibits a resonance scattering peak at 580 nm. It has a new absorption peak at 530nm against the reagent solutions, and the BB concentration c_(BB) in the range of 0～5.5×10^(-5) mol/L is linear to the A_(530) value. Bosed on this result, new spectrophotometric method has been proposed for the determination of berherine in injection samples. The resonance scattering spectra show that the BB^+ combine with I_3^- to form (I_3-BB) association molecule with hydrophobicity. The association molecules automatically aggregate to (I_3-BB)_n association particles. So that, the particles exhibit a resonance scattering peak at 580 nm, and the particle system is in orange-yellow color.