水貂卵母细胞发育过程中脂滴的形态学及组织化学变化的研究

MORPHOLOGICAL AND HISTOCHEMICAL STUDIES ON THE CHANGES OF LIPID DROPLETS IN DEVELOPMENT OF OOCYTES OF THE MINK

应用电镜及光镜组织化学技术,对水貂卵巢內各发育阶段卵母细胞中的脂滴进行了研究。在水貂卵母细胞中,脂滴的形成是卵母细胞生长开始的标志之一。在生长早期,卵母细胞中脂滴分散在皮质与核之间的胞质中,且脂滴的染色较深,在晚期阶段,脂滴明显增大,聚集到一起,染色变浅。经油红O和酸性氧化苏木素染色证明,在卵泡腔形成之前,脂滴主要是由中性脂肪和磷脂组成,在卵泡腔形成之后,脂滴成分主要是中性脂肪,磷脂消失。实验结果表明,脂滴可能是来自于相对静止期卵母细胞中分布的黑色嗜锇颗粒(直径约0.38μm),经过脂质的不断蓄积而形成脂滴。脂滴量特别多是水貂成熟卵母细胞的特点之一。

Mink oocytes of different development stages were studied with an electron microscope and histochemical techniques. In mink oocytes lipid droplet formation is one of the marks that indicates the beginning of growth of oocytes. In early development stage of oocytcs, lipid droplets are scattered in the ooplasm between the cortical region and nucleus and arc stained dark in color. But in the late stage their size increases markcdly.The droplets gather together, and arc stained light in color. Tt was found by staining that lipid droplets consisted mainly of neutral fat and phospholipid before the appcarance of the follicular antrum, but after the appearance they consisted mainly of neutral fat, and phospholipid disappeared. The results of the experiment showed that the lipid droplets came probably from a kind of black osmiophilic granules (with diameter about 0.38μm) in the quiet oocytcs. Possessing a lot of lipid droplets is one of the characteristics of mink's mature oocytcs.