摘要
稻曲病菌在PD 液体培养基中生长良好,并能产生对植物细胞具有高度生物抑制活性的毒素。生物学活性测定袁明,用100%的甲醇能提取稻曲病菌液体培养物中的粗毒素。粗毒素对小麦胚根胚芽的生长有强烈的抑制作用。把毒素主要成分Ustiloxin A 和BSA 偶联后,制备了抗血清,ELISA 检测表明用两种偶联剂偶联所制备的抗体效价分别为1∶20000和1∶6000。进一步的免疫胶体金标记分析表明,所制备的抗体能与茼丝中分泌的毒素特异性结合,说明所获得的抗体是特异性的。
False smut is an increasingly importanl rice disease in recent years,caused by apathogen,Ustilaginoidea virens Cooke,Takahashi.The pathogen fungus grew well in PD medium,and could produce toxins,ustiloxins,which strongly inhibited the growth of radicle and embryo ofplants.The rough ustiloxins were extracted with 100% methanol from the culture filtrate in thestudy.The antiserum against Ustiloxin A,the main component of the toxins,was raised byimmunizing New Zealand rabbit.Elisa assay showed that the titers of the two antiserums obtainedwere 1:20000 and 1:6000,respectively.The specificity of the antiserums was also confirmed byImmuno-gold labeling.
出处
《实验生物学报》
CSCD
北大核心
2004年第4期310-314,共5页
Acta Biologiae Experimentalis Sinica
