摘要
细胞程序性死亡(Programmed Cell Death,PCD)是一种受基因调控的、主动的、连续的程序化反应.一般发生程序性死亡的细胞具有较为典型的形态及生化特征:细胞收缩、核染色质浓缩、趋边化;DNA在一定位点被核酸内切酶降解成以180bp为基数的片段,在琼脂糖凝胶电泳中呈现"梯"状条带(DNA Ladder),即:DNA片段化[1,2]等.近年来,随着植物细胞程序性死亡的研究进展,人们逐渐认识到细胞程序性死亡不但是植物正常发育中必不可少的组成部分[3-5],而且是植物抵抗病原体侵染的重要机制.
The biochemical character of PCD in different interaction between wheat and Puc-cinia recondita f.sp tritici was observed using DNA agarose gel electrophoresis and end labelingin situ (Terminal Deoxynucleotidyl Transferase-mediated dUTP Nick End TUNEL).The DNA lad-der,which is the typical character of programmed cell death(PCD),was observed in incompatiblecombination (cv.lorvin10×race 162) and the positive reaction in wheat leaves of incompatiblecombination since 12h after inoculation also appeared.The results,coupled with the previousstudy,indicated that HR was a typical form of PCD in incompatible combination between wheatand wheat leaf fungus.
出处
《实验生物学报》
CSCD
北大核心
2004年第4期329-332,共4页
Acta Biologiae Experimentalis Sinica
基金
河北省自然科学基金(399172
301124
303180)
河北省教委博士基金资助(B2001211)
关键词
小麦
叶锈菌
细胞
程序性死亡
DNA
基因
Wheat.
Leaf fungus.
Programmed cell death.
DNA ladder.
TUNEL
