猪UCP3基因部分编码区序列分析及其单核苷酸多态与胴体、肉质性状的遗传效应

Sequencing Analysis on Partial Translated Region of Uncoupling Protein-3 Gene and Single Nucleotide Polymorphism Associations with the Porcine Carcass and Meat Quality Traits

以猪解耦联蛋白基因 3(UCP3)作为控制猪胴体与肉质性状主基因的候选基因。利用直接测序法对 4个品种猪骨骼肌中UCP3基因的部分编码区序列 (第 4外显子部分及第 5、6、7外显子全部片段 )进行比较分析 ,发现3个cSNP位点 ,其中ORF中第 84 2碱基的突变可导致相应编码氨基酸序列的改变 :甲硫氨酸→苏氨酸 ,选取此位点作为猪UCP3基因的多态位点。用PCR SSCP检测方法在 3个品种猪中进行该cSNP位点多态性片段的基因型分型 ,结果显示在 3个猪群中表现出 3种基因型 (AA、AB、BB) ,χ2 独立性检验结果表明 3种基因型在各品种间分布不一致 ,梅山猪同大白、长白猪分别比较差异极显著 (P <0 0 1) ;对大白×梅山资源家系F2 代 139头个体进行了该多态片段的基因型鉴定 ,并对其基因型与所检测个体相应的胴体、肉质性状采用GLM分析进行遗传效应研究 ,结果表明 :该基因对一些胴体、肉质性状有显著性影响 ,并且该基因以加性效应为主 (如 ,眼肌高度、背最长肌色值、系水力的加性效应都达显著水平 )。因此 ,推测UCP3基因可能是影响猪胴体及肉质性状的主效基因或与主效基因紧密连锁的标记基因 ,并且能够在分子标记辅助选择中用于对猪胴体。

Porcine uncoupling protein-3 gene was used to study its effect on carcass and meat quality traits.The UCP3 partial translated regions in skeletal muscle from four pig breeds were sequenced and the comparison of the fragment sequences among four pig breeds showed that there were three coding-region single nucleotide polymorphisms (cSNP),of which the mutation at 842 bp of the open reading frame can result in the amino acid chang between methionine and threonine,so we selected the mutation as polymorphic site.The detection of polymorphic fragment by the technique of single strand conformation polymorphism (SSCP) and χ~2 analysis among three pig breeds indicated that the distribution of three genotypes(AA,AB and BB) was significantly different between Meishan and Large White or Meishan and Landrace(P<0.01).In addition,association analysis of UCP3 polymorphism with carcass and meat quality traits was conducted in the F 2 generation from the Large White×Meishan resource family,the results with GLM analysis showed that UCP3 polymorphism has significant effect on several carcass and meat quality traits and also additive effect on some carcass and meat quality traits predominated in the resource family.It implied that UCP3 gene could be a candidate gene locus or a linked marker to a major gene,which affects the porcine carcass traits and meat quality traits significantly.