摘要
由 19头杂种公猪 [皮特兰× (皮特兰×汉普夏 ) ]、5 2头杂种母猪 [Leicoma× (大约克×长白 ) ]及其 332头后代组成的商品群作为参考家系 ,选择 172个微卫星标记和 3个 1类标记 (RYR1、PRKAG3、PIT1)对参考家系的个体进行遗传标记分型 ,构建了猪的整个基因组微卫星连锁图谱。按照线性评分的方法 (竖耳 :1分 ;垂耳 :- 1分 ;半垂耳 :0分 )测定猪的耳型表型值。耳型测定值的平均值为 0 .2 3,标准差为 0 .82。应用最小二乘回归方法对耳型的QTL进行定位 ,结果仅在 6号染色体的末端 (Sw1881和Sw32 2 )之间以 1%基因组显著性水平检测出 1个耳型基因位点 ,而在其他染色体上即使 10 %染色体显著性水平上也没有发现QTL。
A commercial pig population,including 19 hybrid boars [Piétrain × (Piétrain x Hampshire)],52 hybrid sows [Leicoma x (Large White x Landrace)] and their 332 offspring,was used to construct a reference pedigree,with which a linkage map of whole genome was created using 172 microsatellite markers and three type-1 markers (RYR1,PRKAG3,PIT1). The average of the notes of ear shape (pricky ear shape noted by 1; middle ear shape noted by 0; floppy ear noted by -1) detected by a trained technician is 0.23 and the variation 0.82. QTL mapping for ear shape was carried out with the least square regression method. The result showed that only one QTL for ear shape was detected at 1% genomewise level at the end (between Sw1881 and Sw322) of the chromosome 6 and no QTL was found on the other chromosomes even at 10% chromosomewise level.
基金
德国H .WILHELMSCHAUMANN基金会资助~~
关键词
猪
微卫星标记
耳型
QTL定位
pig
microsatellite marker
ear shape
QTL mapping
