摘要
以鳞茎为外植体对两个不同用途的百合品种(食用与鲜切花百合)组织培养和快速繁殖进行了比较研究。结果表明,两种百合对培养基所用的外源激素的要求不同,食用百合品种适宜用MS+6 BA1.0mg/L+2,4 D0.5mg/L诱导的小鳞茎多,花百合品种则用MS+6 BA0.5mg/L+NAA1.0mg/L既能诱导出较多的小鳞茎,又能诱导出质量较好的愈伤组织,愈伤组织再以6 BA0.5mg/L+NAA0.25mg/L培养可以诱导出较好质量的小鳞茎。低浓度的IAA及适当浓度的活性炭有利于提高百合生根。移栽时以根系多、粗壮的小鳞茎移栽成活率高,花百合的移栽成活率比食用百合高。
Squamose bulbs of lily(Lilium brownii F.E.Brown var.viridulum Baker)were used as exoplants to compare the difference of tissue culture and clonal propagation of two lily varieties(one for food and another for flower)by scales in (vitro.)The obtained results of this study showed the adaptable combinations of growth regulator in culture induction medium for two lily varieties were different:6-BA 1.0 mg/L+2,4-D 0.5 mg/L was better for edible variety,6-BA 0.5 mg/L+NAA 1.0 mg/L was better for flower variety and 6-BA 0.5 mg/L+NAA 1.0 mg/L was better for its callus inducing more little bulbs with high guality.Lower concentration of IAA and optimum concentration of active charcoal in the culture medium could help improving rooting of lily.The best induction medium for inducing better quality from callus was 6-BA 0.5 mg/L +NAA 0.25 mg/L.
出处
《浙江农业学报》
CSCD
2004年第4期198-201,共4页
Acta Agriculturae Zhejiangensis
基金
浙江省教育厅资助项目(2000324)
