摘要
蓝舌病病毒非结构蛋白NS_1基因在各型中具有高同源性,可作为群特异性检测的依据。采用NS_1基因中210bp的区段作为PCR扩增模板,经逆转录酶合成第一股cDNA后,再进行PCR扩增。结果对BTV可扩增出特异片段,而鹿流行性出血病病毒和茨城病病毒则不出现扩增。
Non-structure protein (NS1) of bluetongue virus demonstrates high homology in its different serotypes and this can be base for group-specific detection. 210 fragment in NS1 gene was used as template for PCR amplification following its synthesis into the first band cDNA by reverse transcriptase, resulting in specific reaction with bluetongue virus and not with EHDV and Ibaraki virus.
