东方杯叶吸虫囊蚴的体外培养和发育的研究

IN VITRO CULTURE AND DEVELOPMENT OF CYATHOCOTYLE ORIENTALIS (TREMATODA)METACERCARIAE

本文用含小鸡血清的培养基将东方杯叶吸虫脱囊后尾蚴体外培养至怀卵成虫,但虫卵不正常。添加小鸡小肠粘膜浸出液能明显提高怀卵率和产卵量,但不影响怀卵量,41℃比37℃培养结果更好;后尾蚴的发育经历了精巢、阴茎囊、卵巢和卵黄腺的形成及怀卵和产卵的过程,体外培养中虫体发育延缓。

A variety of media were used to culture undifferentiated metacercaria of Cyathocotyle orientals (Faust, 1922) to maturity in vitro. Excysted metacercariae survived about 5 - 16 days and continued their somatic growth, but their genitalia failed to develop further in the media supplemented with foetal or bovine calf serum, egg yolk, album or whole egg macerate. Ovigerous adults were obtained by day 4 only in the medium NCTC 135 plus 40% chicken serum, but eggs produced in vitro were abnormal in appearence and failed toembry-onate. The rate of oviposition and the percentage of flukes with eggs were increased remarkably when 20% chicken small intestine mucosal extracts were supplemented. Other optimal physical factors were an ambient temperature of 41 ℃ and a gas phase of 5% CO2.The development of the metacercariae in vitro was compared with that in chicken. The developmental process in vivo and in vitro was arbitrarily divided into six stages: formation of testes, cirrus sac and ovary, vitellgensis ,ovigerous adult and oviposition. In the process, there was an initial lag phase followed by a rapid development. Growth rate of the flukes in vivo was higher than that of the flukes cultured in vitro.The role of some supplements and development of C. orientalis in vivo and in vitro were discussed.