肺表面活性物质对全氟异丁烯吸入性肺损伤的预防效果

Protective effect of pulmonary surfactant on acute lung injury induced by perfluoroisobutylene inhalation in mice

目的 通过观察外源性肺表面活性物质 (pulmonarysurfactant,PS)对小鼠全氟异丁烯 (perfluoroisobutylene ,PFIB)吸入性肺损伤的防护效果 ,以期寻找预防PFIB中毒的有效药物。方法 小鼠PFIB染毒前 2h经气管给予不同剂量的PS制剂 (含二棕榈酰磷脂酰胆碱 ,dipalmitoylphosphatidylcholine ,DPPC)。采用静式全暴露法染毒PFIB 5min ,观察 48h内给药组与对照组小鼠的活存率、肺含水量和支气管肺泡灌洗液 (bronchoalveolarlavagefluid ,BALF)中蛋白含量。结果 预防给予 3个剂量的PS制剂 (DPPC 2 5mg/kg、 5 0mg/kg和 10 0mg/kg)均能显著提高PFIB中毒小鼠的存活率 (P <0 0 1) ;DPPC 5 0mg/kg剂量组 ,在中毒后 16h和 2 4h小鼠肺含水量明显低于生理盐水对照组 (P <0 0 5 ) ,在中毒后 16hBALF中总蛋白和白蛋白的含量显著低于生理盐水对照组 (P <0 0 1)。

Objective To find a successful prophylactic intervention for the acute lung injury exposed to perfluoroisobutylene(PFIB) by testing the exogenous pulmonary surfactant.To looking for an effective measure to protect against PFIB poisoning by observing the effect of pulmonary surfactant on acute lung injury caused by PFIB inhalation in mice.Method Three dosages of pig pulmonary surfactant were intratracheal administration to mice 2 h before gaseous PFIB exposure for 5 minutes.The mortality,pulmonary water content and bronchoalveolar lavage fluid from the test groups were detected.Result All dosages of PS could significantly lower the mortality(P<0.01)compared with control group.The pretreated group with PS(50 mg/kg)could significantly lower the pulmonary water content at 16 h and 24 h after poisoning (P<0.05),and also could significantly lower protein concentration in BALF at 16 h after poisoning(P<0.01)compared with control group.Conclusion The exogenous PS has some protective effects on acute lung injury induced by PFIB.