乙羧氟草醚农药对大鼠肝脏毒性的研究

Hepatoxicity of fluoroglycoften-ethyl pesticide in rats

为探讨乙羧氟草醚农药对大鼠肝脏的毒性作用 ,将 80只 6周龄Wistar大鼠 ,按体重随机分为 4组 ,每组 2 0只。分别以含有乙羧氟草醚原药 0、 2 0 0、 10 0 0及 5 0 0 0mg/kg饲料饲喂 13周。每周 1次称量大鼠体重、摄食量 ,饲养结束后 ,检测大鼠血清丙氨酸氨基转移酶 (ALT)、天门冬氨酸氨基转移酶 (AST)、碱性磷酸酯酶 (ALP)水平 ,测定肝脏质量并计算肝脏系数 ,光镜下对大鼠肝脏进行组织病理学检查。结果表明 ,高浓度组大鼠体重增长明显受到抑制 ,摄食量明显减少 ,雌、雄鼠血清ALT、AST及雄鼠血清ALP水平明显升高 ,雌鼠血清ALP水平也有升高趋势。肝脏系数增大 ,并出现组织病理学改变。中、低浓度组大鼠与对照组相比 。

To study the effects of fluoroglycoften-ethyl pesticide on the liver,Wistar rats at 6 weeks old with weight of 80～90 grams were randomly divided into four groups,with 20(10 males and 10 females) in each group.The rats were orally administrated with fluoroglycoften-ethyl at doses of 0,200,1 000 and 5 000 mg/kg,respectively,for 13 weeks.Body weight and food consumption were measured once a week.Serum activities of alanine aminotransferase(ALT),aspartate aminotransferase(AST)and alkaline phosphatase(ALP)were determined at the end of the study.Liver weight was measured and liver index calculated.Pathological examination for the liver was also performed with light microscopy.Results showed that body weight was obviously inhibited and food ingestion reduced in the rats with high-dose of fluoroglycoften-ethyl,as compared with the control rats.Serum activities of ALT and AST increased significantly in male and female exposed rats.Serum activity of ALP increased significantly only in male rats,but appeared an increasing trend also in female rats.Liver index increased significantly and pathological changes of the liver were also observed with light microscopy in the high-dose exposed rats.No significant changesy in indicators were found in the middle and low exposed rats,as compared to control rats.