摘要
A method was developed to quickly determine genistin, genistein, kaempferol and rutin in Fructus sophorae by β -CDmodified capillary zone electrophoresis. Based on the investigation of the effects of buffer pH and concentration, β -CD concentration,SDS concentration, capillary temperature, the analytical conditions were optimized. Under the optimized conditions, the three analytes were well separated in less than 6 min. A good linear relationship between the peak area and concentration was found in the 2 ～ 400g/mL,2 ～ 300g/mL 2 ～ 200g/mL and 5 ～ 500g/mL concentration range for genistin, genistein, kaempferol and rutin, respectively. The detection limits were 0.8g/mL, 0.6g/mL, 0.6g/mL and 1.4g/mL for genistin, genistein, kaempferol and rutin, respectively.
A method was developed to quickly determine genistin, genistein, kaempferol and rutin in Fructus sophorae by β-CD modified capillary zone electrophoresis. Based on the investigation of the effects of buffer pH and concentration, β-CD concentration, SDS concentration, capillary temperature, the analytical conditions were optimized. Under the optimized conditions, the three analytes were well separated in less than 6 min. A good linear relationship between the peak area and concentration was found in the 2-400g/mL, 2-300g/mL 2-200g/mL and 5-500g/mL concentration range for genistin, genistein, kaempferol and rutin, respectively. The detec-tion limits were 0.8g/mL, 0.6g/mL, 0.6g/mL and 1.4g/mL for genistin, genistein, kaempferol and rutin, respectively.
出处
《生命科学仪器》
2004年第3期30-30,32,共2页
Life Science Instruments
基金
"十五"科技攻关重大项目(2001BA210A04-7)
国家自然科学基金(20175025)
国家自然科学基金分析化学重点基金(20235010)
电分析化学国家重点实验室基金
"九五"科技攻关重点项目(96-A23-01-06)的资助
