HPLC法测定咳喘口服液中黄芩苷的含量

Determination of baicalin in Kechuan oral liquid by HPLC

目的 :建立咳喘口服液中黄芩苷的含量测定方法。方法 :用高效液相色谱法测定 ,DiamonsilC18色谱柱 (2 5 0mm× 4 .6mm) ,流动相 :ψ(甲醇 ∶水∶磷酸 ) =4 5 ∶ 5 5∶0 .2 ,检测波长 2 80nm。结果 :黄芩苷对照品在 0 .10 12～ 1.0 12mg/mL范围内与峰面积呈良好线性关系 ;精密度、重复性试验结果RSD分别为 0 .4 2 %和 0 .76 % ;平均回收率为 10 0 .4 7% ,RSD 0 .95 %。结论 :本法简便、灵敏、准确、重现性好 。

Objective:To establish the content determination method of baicalin in Kechuan oral liquid by HPLC.Method: The analytical column was Diamonsil C 18 (250 mm×4.6 mm), ψ(methanol∶water∶phosphoric acid)=45∶55∶0.2 as mobile phase.The detection wavelength was 280nm. Result:The linear range was 0.1012～1.012 mg/mL. The RSD of the accuracy and repeatability experiment were 0.42% and 0.76% respectively. The average recovery of 100.47% with RSD=0.95%. Conclusion: The established method is convenient, sensitive, and accurate, which can be used as standard of quality control for Kechuan oral liquid.