恶性黑色素瘤中C-erbB-2、p53、p16蛋白的表达

EXPRESSION OF C-erbB-2,p53 AND p16 PROTEINS IN MALIGNANT MELANOMA

目的:探讨恶性黑色素瘤中C蛳erbB蛳2、p53、p16蛋白的表达及其相互关系。方法:应用免疫组化S蛳P法对14例恶性黑色素瘤和39例色素痣组织中C蛳erbB蛳2、p53、p16蛋白表达进行检测。结果:14例恶性黑色素瘤中C蛳erbB蛳2、p53和p16蛋白的阳性率分别为50 %(7/14)、64 %(9/14)、43 %(6/14)。39例色素痣中C蛳erbB蛳2、p53和p16蛋白的阳性率分别为18 %(7/39)、36 %(14/39)、77 %(30/39)。C蛳erbB蛳2、p53、p16蛋白阳性率与恶性黑色素瘤的发生有密切的关系(P < 0.05)。结论:肿瘤多基因分析比单基因分析有价值。癌基因C蛳erbB蛳2和抑癌基因p53、p16蛋白的表达异常及协同作用在恶性黑色素瘤的发生发展中起重要作用。

Objective: To investigate the expression of C- erbB- 2, p53 and p16 proteins in malignant melanoma and to analyze their correlations. Methods: A immunohistochemical S- P methods was used to detect the C- erbB- 2, p53 and p16 gene expression in 14 cases of malignant melanoma and 39 cases pigmented nevers. Results: Positive rates of C- erbB- 2, p53 and p16 in 14 cases of malignant melanoma were 50 %, 64 %, 43 %. 39 cases of pigmented nevers were 18 %, 36 %, 77 % respectively. C- erbB- 2, p53, p16 gene expression were related (P <0.05). Conclusion: The analysis of multiple oncogene and/or antioncogene is more valuable than simple oncogene and/or antioncogene. The altered expression of oncogene C- erbB- 2 and antioncogenes p16 and p53 might play a promotive role in the tumorgenesis and development of malignant melanoma.