肾通注射液对急性肾衰竭家兔肾血流量的影响

Effect of Shentong Injection on Renal Hemodynamics in Rabbits with Acute Renal Failure

目的 :评估肾通注射液对急性肾衰竭 (简称ARF)家兔肾血流量的影响 ,并初步探讨其机制。方法 :健康雄性大耳白兔 4 0只 ,随机分为正常组、模型组、肾通治疗组和丹参治疗组 (每组各 10只 )。后 3组用 5 0 %甘油生理盐水 15ml·kg-1在双后下肢肌肉注射 ,分别于造模后第 2 4 ,4 8和 72h观察各组肾血流动力学、肾功能及血、肾局部内皮素 (ET 1)、一氧化氮 (NO)的变化。结果 :与正常组相比 ,模型组肾血流量 (RBF)和内生肌酐清除率 (Ccr)明显降低 (P <0 .0 1) ,血浆和肾局部ET 1上升 ,NO下降 ,有显著性差异 (P <0 .0 1)。肾通组RBF和Ccr明显高于模型组 ,肾局部ET 1明显降低 ,同时NO上升。肾通组与丹参组相比 ,疗效无明显差别 (P >0 .0 5 )。结论 :肾通注射液可增加实验性ARF家兔的肾脏血液供应 ,改善微循环 ,提高RBF和Ccr,保护肾功能 ,调节ET 1和NO水平 ,而ET 1和NO水平改变是肾通注射液的重要作用机制之一。

Objective: To investigate the effects of Chinese traditional medicine Shentong injection on renal hemodynamics in rabbits with acute renal failure (ARF) and to explore its mechanisms. Methods: Forty male rabbits were randomly divided into four groups,10 rabbits in each: control group(N)、model group(M)、Shentong injection treated group(T)and angelica injection treated group(D). In M, T, D groups,50% glycerin physiologicalsaline (15 ml·kg -1 ) were injected in lower extremities. 24,48 and 72h after glycerin-induced ARF were made,renal hemodynamics were measured by color Doppler ultrasound,renal functional parameters were monitored by biochemical method,plasma and renal local endothelin-1 (ET-1) and nitric oxide (NO) were investigated respectively. Results: Compared with normal group,renal blood flow (RBF) and creatinine clearance rate (Ccr) were remarkably reduced in model group ( P< 0.01),plasma and renal local ET-1 were increased while NO decreased,the differences were significant ( P< 0.01). In Shentong treated group,RBF and Ccr were higher significantly than those in M group,renal local ET-1 decreased evidently while NO increased. Compare with D group,there were no significant in difference T group ( P> 0.05). Conclusion: Shentong infection can relax renal vascularity of ARF,relief the resistence of microcirculation,improve RBF and Ccr,and protect renal function through the mechanisms of the changes of ET-1 and NO .