小檗碱对高脂血症伴胰岛素抵抗大鼠糖脂代谢的影响

Effect and mechanisms of berberine on hyperlipidemic and insulin resistant rats

目的 :观察小檗碱对高脂血症伴胰岛素抵抗 (IR)大鼠糖脂代谢的影响 ,探讨其调脂和改善IR的机理。方法 :通过喂养高脂饲料和小剂量静注链脲佐菌素 (STZ)建立高脂血症伴IR大鼠模型 ,测定各不同处理组血糖、脂、胰岛素水平和脂代谢关键酶活性。结果 :小檗碱组大鼠总胆固醇 (TC)、甘油三酯 (TG)、载脂蛋白B(ApoB)、低密度脂蛋白 胆固醇 (LDL C)、游离脂肪酸 (FFA)均比模型组明显降低 (Ρ <0 .0 5或Ρ <0 .0 1) ,而高密度脂蛋白 胆固醇 (HDL C)、载脂蛋白A(ApoAⅠ )、胰岛素敏感指数 (ISI)显著升高 ;血脂蛋白脂酶 (LPL)活性显著升高 (Ρ <0 .0 5或Ρ <0 .0 1)。结论 :小檗碱可能通过降低血FFA而改善IR ,其调脂功效可能与升高LPL活性有关。

OBJECTIVE To study the effects of berberine on lipids and glucose metabolism in hyperlipidemic and insulin resistant (IR) rats and to explore the correlated mechanisms. METHODS The murine model of hyperlipidemia and IR was established by feeding with high fat laboratory chow and intravenously injecting streptozotocin. The serum glucose and insulin level, serum lipids profile, activity of lipoprotein lipase in each different treated group were determined. RESULTS It was demonstrated that murine serum total cholesterol (TC), triglycerides (TG), apolipoprotein B (ApoB), low density lipoprotein-cholesterol (LDL-C), free fatty acid (FFA) in berberine treated group were significantly decreased as compared with those in model group (Ρ< 0.05 or P< 0.01), while the level of serum high density lipoprotein-cholesterol (HDL-C), insulin sensitive index (ISI), apolipoprotein A (ApoAⅠ), the activity of lipoprotein lipase in berberine treated group obviously elevated (Ρ< 0.05 or Ρ< 0.01). CONCLUSION It is suggested that the administration of berberine in hyperlipidemic and insulin resistant rats can decrease blood FFA level and raise the activity of lipoprotein lipase, which may lead to the amelioration of blood lipid and glucose metabolism.