泌尿生殖道沙眼衣原体临床检测方法的比较

Comparison between detection methods for chlamydia trachomatis infecting genitourinary tract

目的探讨几种检测泌尿生殖道沙眼衣原体感染方法的临床诊断价值。方法免疫荧光法,糖原试验,胶体金标记技术免疫检测,聚合酶链反应(PCR)分别对100例妇产科就诊患者宫颈分泌物标本进行平行检测。结果免疫荧光法阳性检测率为23%,糖原试验阳性检测率为26%,胶体金标记技术免疫检测阳性检测率为22%,PCR 法阳性检测率为34%。结论四种试验方法均可用于沙眼衣原体感染的诊断,可选择两种或两种以上方法平行进行检测,以提高诊断准确率。

Objective To evaluate clinical diagnostic merits of severa| detection methods for chlmydia trachomatisInfecting gonitourinang tract.Methods Immunofluorescence,glycogen test,gold labeling immunoassay and polymerase chain reaction (PCR)were used to detect specimen of cervical secretion from 100 women.Results Positine rate of immunofluorecence was 23%;that of glycogen test was 26%;that of gold labelingimmunoassay was 22%;that of PCR was 34%.Conclusion The four methods may all he used to detect infection of chlamydia trachomatis,however,it is recommended that two detection methods be used in parallel to improve accurarcy of diagnosis.