内源性一氧化碳对高血压大鼠血压的长期影响

Long-term influence of endogenous carbon monoxide on blood pressure of hypertensive rats

目的 :探讨内源性一氧化碳对高血压大鼠的长期影响及其可能机制。方法 :3 2只Wistar大鼠随机分为 4组 ,A组为对照组 ;B组为高血压组 ;C和D组为高血压干预组 ,C组用Hm ,D组用Hm +HLL干预。测量大鼠尾动脉收缩压。检测血一氧化碳 ,ET 1,NO质量浓度。结果 :A组血压在实验过程中无明显变化 ;B ,C ,D组血压均较A组明显升高 (P均<0 .0 1) ;药物干预后C ,D组血压较B组降低 ,D组血压低于C组 (P均 <0 .0 1)。血NO质量浓度B ,C ,D组较A组降低 ,D组较B ,C组升高 (P均 <0 .0 1)。血CO质量浓度C ,D组较A ,B组升高 ,D组高于C组 (P均 <0 .0 1)。B ,C组的ET 1质量浓度较A ,D组升高 (P均 <0 .0 5 )。结论 :内源性一氧化碳能长期有效地降低高血压大鼠的血压 ,可能与其能舒张血管平滑肌及升高NO、降低ET 1质量浓度有关。

Objective To investigate the long-term influence of endogenous carbon monoxide(CO) on blood pressure of hypertensive rats and its possible mechanism. Methods Thirty-two Wistar rats were randomly divided into control group(A), hypertensive group(B), Hm interventional group(C) and Hm+HLL interventional group(D). Blood pressure was measured. The concentration of CO, NO, and ET-1 was measured in the 10th week. Results Blood pressure was increased in group B, and was lower in group C and D than in group B(P<0.01). The concentration of CO in group C and D were higher than that in group A and B(P<0.01), and was higher in group D than in group C(P<0.01). The concentration of NO in group B, C and D were lower than that of group A(P<0.01), and higher in group D than in group B and C(P<0.01). The concentration of ET-1 was increased more in group B and C than in group A and D(P<0.05). Conclusion Endogenous carbon monoxide can effectively decrease blood pressure of hypertensive rats, which may be related to the effect of dilating vascular smooth muscle and increasing NO concentration and decreasing ET-1 concentration mediated by CO.