3MICHAEL R STONER, DOUGLAS A DALE, PETER J GUALFETTI et al. Protease autolysis in heavy - duty liquid detergent formulations effects of thermodynamic stabilizers and protease inhibitors [ J ] Enzyme and Microbial Technology ,2004,34 (2) : 114 - 125.
4LOWRY O H,ROSEBROUGH N J,FARR A L,et al. Protein measure- ment with the folin phenol reagent[ J]. J Biol Chem, 1951,193 ( 1 ) : 265 - 275.
5Sierkstra L N, Klugkist J, Markvardsen P, et aL Protease variants and coropositions[P}. US 20110230386 A1, 2011-09-22.
6Acevedo J, Rodriguez V, Saavedra M, et al. Cloning, expression and decoding of the cold - adaptation of a new widely represented thermolabile subtilisin- -like protease[J]. Journal of Applied Microbiology, 2013, 114(2): 352-363.
7Stmu.sberg S L, Ruan B, Fisher K E, et al. Directed coevolution of stability and catalytic activity in calcium-free subtilisin[J]. Biochemistry, 2005. 44: 3272-3279.
8Li Z, Roccatano D, Lorenz M, et al. Directed evolution of Subtilisin into a highly active and guanidiniuro chloride - and sodium dodecyl.sulfate- -tolerant protease[J]. Chem Bio Chem, 2012, 13: 691-699.
9Martinez tk, Jakob F, Tu R, et al. Increasing activity and them,al resistance of bacillus gibsonii alkaline protease (BgAP) by directed evolutionU]. Biotechnology and Bioengineering, 2013, 110(3) :711-720.
10Schroeder M, Lenting H B M, Kandelbauer A, et al. Restricting detergent protea.se acdon to surface of protein fibres by chemical modification[J]. Applied Microbiology and Biotechnology, 2006, 72: 738-744.
