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表皮生长因子对MDPC-23细胞增殖和ALP活性的影响 被引量：1

The effect of 1,25-(OH)_2D_3 on the proliferation and ALP activity of MDPC-23 cells
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摘要 目的 :观察表皮生长因子 (epidermalgrowthfactor ,EGF)对成牙本质细胞系MDPC -2 3细胞增殖和碱性磷酸酶 (alkalinephosphatase ,ALP)活性的影响。 方法 :细胞培养、MTT比色测定法和碱性磷酸酶活性测定法。结果 :在一定浓度范围内 ,EGF明显促进MDPC -2 3细胞增殖 ,抑制MDPC -2 3细胞内碱性磷酸酶活性 ,并呈一定的剂量依赖性。结论 :EGF能促进成牙本质细胞增殖 。 Objective:To examine the effects of epidermal growth factor(EGF) on the proliferation and alkaline phosphatase (ALP)of odontoblast cell line MDPC-23. Method:Cell culture, MTT colorimetric assay and assay of ALP activity were used.Result:EGF significantly promoted the proliferation of MDPC-23 cells, while inhibited greatly ALP activity of cells in a dose-dependent manner. Conclusion: EGF could inhibit the proliferation, and promote the differentiation of odontoblast cells.
作者 刘晗 何文喜
机构地区 第四军医大学口腔医学院牙体病科
出处 《临床口腔医学杂志》 2004年第7期399-400,共2页 Journal of Clinical Stomatology
关键词 1.25-二羟维生素D3 增殖 碱性磷酸酶 分化 epidermal growth factor proliferation alkaline phosphatease differentiation
分类号 Q813.1 [生物学—生物工程]
  • 相关文献

参考文献8

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二级参考文献2

  • 1赵大为.维生素D与牙齿发育的关系[J].牙体牙髓牙周病学杂志,1996,6(3):189-191. 被引量：3
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共引文献1

同被引文献11

  • 1胡静,谭颖徽,何飞,张萍.IGF-1、bFGF对人牙髓干细胞的体外诱导分化的影响[J].第三军医大学学报,2006,28(8):778-780. 被引量：4
  • 2张光东,徐艳,吴友农,刘卫红,朱玲,俞未一.碱性成纤维细胞生长因子和胰岛素样生长因子-1对人牙髓细胞碱性磷酸酶活性及矿化能力的影响[J].口腔医学,2006,26(4):261-263. 被引量：7
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临床口腔医学杂志
2004年 第7期

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