摘要
目的 :观察表皮生长因子 (epidermalgrowthfactor ,EGF)对成牙本质细胞系MDPC -2 3细胞增殖和碱性磷酸酶 (alkalinephosphatase ,ALP)活性的影响。 方法 :细胞培养、MTT比色测定法和碱性磷酸酶活性测定法。结果 :在一定浓度范围内 ,EGF明显促进MDPC -2 3细胞增殖 ,抑制MDPC -2 3细胞内碱性磷酸酶活性 ,并呈一定的剂量依赖性。结论 :EGF能促进成牙本质细胞增殖 。
Objective:To examine the effects of epidermal growth factor(EGF) on the proliferation and alkaline phosphatase (ALP)of odontoblast cell line MDPC-23. Method:Cell culture, MTT colorimetric assay and assay of ALP activity were used.Result:EGF significantly promoted the proliferation of MDPC-23 cells, while inhibited greatly ALP activity of cells in a dose-dependent manner. Conclusion: EGF could inhibit the proliferation, and promote the differentiation of odontoblast cells.
出处
《临床口腔医学杂志》
2004年第7期399-400,共2页
Journal of Clinical Stomatology