重组人β-趋化因子RANTES抗T嗜性HIV-1作用的研究

Study on effect of recombinant RANTES against T-cell-tropic HIV-1 strain

目的 检测重组人β 趋化因子RANTES(RegulationuponactivationnormalT cellexpressedandsecreted)对T嗜性HIV 1病毒株SF 33在T细胞中复制的影响。方法 在SF 33感染细胞前 1h ,用不同浓度 (5ng/ml、5 0ng/ml、5 0 0ng/ml)的重组人RANTES、GST RANTES或TEP RANTES对MT 4细胞进行预处理。病毒感染后第6天 ,以ELISA方法检测p2 4抗原水平 ,MTT比色法检测感染细胞的存活数。结果 在不同浓度下 ,天然RANTES及修饰RANTES对SF 33p2 4水平不产生显著影响 ,半数抑制浓度IC50 >5 0 0ng/ml,对感染细胞的保护率在 0以下。结论 T嗜性HIV 1病毒株SF 33对RANTES及其修饰蛋白的抑制作用不敏感。

Objective To study the effect of recombinant RANTES(regulation upon activation normal T-cell expressed and secreted)beta-chemokine on replication of T-cell-tropic strain SF-33.Methods The MT-4 cell line was treated for one hour with recombinant RANTES,GST-RANTES and TEP-RANTES(5ng/ml,50ng/ml and 500ng/ml)before infection with SF-33.Six days after infection,p24 level was determined by ELISA,and the surviving infected cells were counted by MTT colorimetric assay.Results The replication of SF-33 was not affected by the treatment of different concentrations of RANTES and its derivatives(50% of inhibitory concentration,(IC 50 >500ng/ml).And the protective rate was below 0.Conclusion The T-cell-tropic HIV-1 SF-33 was insensitive to the antiviral effect of RANTES and its derivatives.