TRAIL诱导白血病细胞凋亡的分子机制研究

MOLECULAR MECHANISM OF APOPTOSIS OF LEUKAMIC CELLS INDUCED BY SOLUBLE TRAIL

目的 检测TRAIL引起的T淋巴白血病细胞凋亡及与之相关的蛋白和信号通路 ,为探索T淋巴细胞凋亡分子机制打下基础 ,为相关肿瘤的治疗提供依据。方法 用TRAIL( 1μg/ml)刺激Jurkat细胞 3 6h以诱导细胞发生凋亡 ,用流式细胞术和MTS比色法检测细胞存活率 ,计算细胞凋亡率 ;用免疫印迹 (Westernblot)检测NF κB的表达和胱天肽酶 (caspase) 3、cas pase 8的变化。 结果 TRAIL能诱导Jurkat细胞凋亡并伴随NF κB表达增加及caspase 3和caspase 8的活化。 结论 TRAIL诱导的T淋巴白血病细胞凋亡的分子机制涉及NF κB及caspase 3、caspase 8,为TRAIL用于治疗白血病提供实验基础。

S Objective: To investigate the signal pathway and expression of related proteins in apoptosis of T lymphocyte (leukemia) induced by sTRAIL, providing a novel insight and information in the apoptosis signaling pathways induced by TRAIL and an important implication for the clinic therapy of T-lymphocyte leukemia. Methods: Jurkat cells treated with TRAIL (1 μg/ml) for 36 h were used to detect the viability with MTS assay and Flow Cytometry, and to detect caspase-8, caspase-3 and NF-κB expression with Western blot. Results: TRAIL could induce apoptosis of Jurkat cells with activation of caspase-8, caspase-3 and increasement of NF-κB expression. Conclusions: Molecular mechanism of apoptosis of T-lymphocyte leukemia induced by TRAIL may involve caspase-8, caspase-3 and NF-κB may be involved in signal pathways in Jurkat cells treated by TRAIL.