摘要
目的探讨基质金属蛋白酶鄄1(MMP鄄1)、金属蛋白酶组织抑制因子鄄1(TIMP鄄1)在心肌炎小鼠心肌胶原重构中的作用。方法用VG染色和图像分析、原位杂交方法观察病毒性心肌炎小鼠及同龄的对照小鼠各时期心肌组织胶原改变及MMP鄄1mRNA、TIMP鄄1mRNA的表达。结果与对照组比较,感染后第14天心肌组织胶原增加不明显,感染后第28天心肌组织血管周围胶原面积(PVCA)显著增加(P<0.001),感染后第56天PVCA及心肌胶原容积分数(CVF)均显著增加(P<0.001)。感染后第7天心肌MMP鄄1mRNA呈现一过性表达;感染后第7天可见TIMP鄄1mRNA表达,第14～21天最为明显,此后减弱,直到第56天。结论TIMP鄄1mRNA表达上调可能在病毒性心肌炎心肌胶原重构中起着重要作用。
Objectives To examine the effect of matrix metalloproteinases-1(MMP-1),tissue inhibitor of metalloproteinases-1(TIMP-1) on myocardial collagen remodeling in a murine model of myocarditis induced by CVB3m. Methods Myocardial collagen was stained with VG and analyzed using a computer assisted procedure on every time point of samples of experimental virus myocarditis mice and the age-matched controls, and the mRNA expression of MMP-1 and TIMP-1 were detected in situ hybridization at the same time. Results The myocardial fibrillar collagen of infected mice was not obviously accumulated on days 14, but on days 28 a significant increase of perivascular collagen area was observed in myocardium(P<0.001), and on days 56 perivascular collagen area and collagen volume fraction in myocardium was obviously increased(P<0.001). The expression of MMP-1 mRNA was transient only on day 7 after infection. The expression of TIMP-1 mRNA was detected on day 7 and was most significant on days 14～21, attenuated thereafter till day 56 after infection. Conclusions The up-regulation of TIMP-1 mRNA expression may contributes to myocardial collagen remodeling in myocarditis mice.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2004年第4期333-335,共3页
Chinese Jouranl of Endemiology
基金
黑龙江省卫生厅科研基金资助项目(2000-048)
