葛根芩连汤的HPCE指纹图谱研究

HPCE fingerprinting of Gegen Qinlian Decoction

目的 采用高效毛细管电泳法 ( HPCE)建立葛根芩连汤的指纹图谱。方法 电解质溶液由 30 mm ol/ L磷酸盐和 4 0 m mol/ L硼酸盐组成 ,未涂层融硅毛细管 ( 6 5 cm× 5 0μm,有效长度 4 3cm ) ,分离电压 2 2 k V,进样 1s,检测波长 2 5 4 nm ,温度 30℃。结果 上述条件下 ,葛根芩连汤中 2 7个成分得到了较好的分离 ,方法学考察结果符合定量测定和定性研究的要求。结论 该方法具有较好的分离效果和良好的精密度 。

Object To study the HPCE fingerprinting of Gegen Qinlian Decoction (GQD). Methods A buffer was composed of 30 mmol/L sodium phosphate and 40 mmol/L borate solution. Capillary electrophoresis was performed using a 65 cm (43 cm to detector) ×50 μm fused-silica capillary tube. Separation voltage was 22 kV, sampling time was 1 s, detected wavelength was 254 nm, and the temperature was maintained at 30 ℃. Results The 27 components in GQD were successfully separated. The observation of methodology was in keeping with quantitatine determination and qualitative study. Conclusion This method can be used for the quality control of the preparation of GQD with good precision.