蚕砂中叶绿素类金属配合物清除活性氧作用研究

Scavenging effect on active oxygen of metal chlorophylls from silkworm excrement

目的 研究蚕砂中提取、精制叶绿素类金属配合物 [Fe2 +( )、Cu2 +( )、Mn2 +( )、Co2 +( ) ]作为抗活性氧 ( O÷2 、H2 O2 、OH· )模拟酶。方法 核黄素—蛋氨酸光照测其清除 O÷2 作用 ,H2 O2 氧化维生素 C法测其催化H2 O2 的分解 ,Fenton-苯甲酸钠荧光法测其对 OH·清除作用 ,小鼠肝匀浆法测其抗脂质过氧化作用。结果 1×10 - 5～ 1× 10 - 6 m ol/ L具有良好的清除 O÷2 作用 ,活性顺序为 :Cu2 +>Mn2 +>Co2 +>Fe2 +;约 1× 10 - 7mol/ L具有分解 H2 O2 作用 ,活性顺序为 :Cu2 +>Mn2 +>Fe2 +>Co2 +;约 1× 10 - 8mol/ L 具有清除 OH·的功能 ,活性顺序为 :Cu2 +>Co2 +>Fe2 +>Mn2 +;约 1× 10 - 8m ol/ L 可使脂质过氧化产物明显减少 ,活性顺序为 :Cu2 +>Co2 +>Mn2 +>Fe2 +。结论 蚕砂中叶绿素类金属配合物可作为抗多种活性氧 ( O÷2 、H2 O2 、OH· )

Object Four metal chlorophylls produced by chlorophyll extracted from silkworm excrement were used as varied function mimicry of anti-active oxygen. Methods The first function, scavenging O ÷ 2, has been proved by using riboflavine-methionine photoreduction methods. The second function, scaveging H 2O 2, has been demonstrated by using the oxidating Vc. The third function, scavenging OH· , has been demonstrated by using Fenton reaction. The complexes were measured by the liver homogenate technique of mice. Results Four model compounds could scavenge O ÷ 2 in the concentration of 10 -5 —10 -6 mol/L and the activity order was as follows: Cu 2+ >Mn 2+ >Co 2+ >Fe 2+ . All could decompose H 2O 2 in the concentration of 1×10 -7 mol/L and the activity order was: Cu 2+ >Mn 2+ >Fe 2+ >Co 2+ . All could scavenge OH· in the concentration of 1×10 -8 mol/L and the activity order was: Cu 2+ >Co 2+ >Fe 2+ >Mn 2+ . All showed that they had obvious action of decreasing the lipid peroxidation in the concentration of 1×10 -8 mol/L. The activity order of anti-lipid peroxidation of complexes was as follows: Cu 2+ >Co 2+ >Mn 2+ >Fe 2+ . Conclusion All above-mentioned complexes were considered to be qualified mimic enzymes of anti-active oxygen.