铁皮石斛与绞股蓝原生质体融合的初步研究

Protoplast fusion in Dendrobium candidum and Gynostemma pentaphyllum

目的 探索铁皮石斛与绞股蓝原生质体杂交融合 ,为中药材的改良提供新途径。方法 两种原生质体通过PEG法进行成对融合 ,融合子培养在添加 BA2 mg/L 及 NAA1m g/L 改良的 MS液体培养基中。结果 分离得到了高产量、活力强、纯度高的铁皮石斛及绞股蓝叶肉原生质体。结论 融合获得了有活力的杂种细胞 ,培养 3d后 ,杂种细胞开始分裂 ,共进行了

Object To explore the protoplast fusion of Dendrobium candidum Wall ex. Lindl. and Gynostemma pentaphyllum. (Thunb) Makino. Methods The two kinds of protoplasts were fused by PEG method, and then cultured in the modified liquid medium containing 2 mg/L BA and 1 mg/L NAA. Results High yield, viability, and pure mesophyll protoplasts were isolated from D. candidum and G. pentaphyllum. Conclusion The first cell division occurres within three days after fusion. And some of the cells are divided three times.