肉苁蓉药材与盐生肉苁蓉培养细胞的主要成分对比研究

Comparison of main constituents of Herba Cistanche and cultured cells of Cistanche salsa

目的 研究肉苁蓉药材与盐生肉苁蓉培养细胞的苯乙醇苷类成分差异。方法 利用傅里叶变换红外光谱(FTIR)和 HPL C技术进行成分对比分析。结果 FTIR的分析结果表明 ,盐生肉苁蓉培养细胞的红外指纹谱图与肉苁蓉药材具有一定相似性 ;HPL C的分析结果表明 ,盐生肉苁蓉细胞中所含的主要成分和肉苁蓉药材相似 ,只是含量有差异。结论 从整体上看 ,盐生肉苁蓉培养细胞中所含成分种类较多 ,且大部分含量较高 ,其中洋丁香酚苷(类叶升麻苷 )

Object To study the difference of phenylethanoid glycoside between the cultured cells of Cistanche salsa (C. A. Mey.) Benth. et Hook. f. and Herba Cistanche. Methods Making comparison by means of FTIR and HPLC technique. Results Using the FTIR technique, the infrared spectrum of cutured cell of C. salsa was similar to that of native Herba Cistanche at a certain extent. The HPLC analysis indicated that the main chemical constituents in cultured cells of C. salsa were similar to that of native Herba Cistanche while considerable difference of constituents was found between them. Conclusion On the whole, there are much more constituents in the cultured cells of C. salsa comparing with the native Herba Cistanche. The content of echinacoside and acteoside in the cultured cells is much higher than that in the native Herba Cistanche.