摘要
研究卡铂损伤灰鼠 (Chinchilla)耳蜗内毛细胞 (Innerhaircell,IHC)后 ,耳蜗核 (CochlearNucleus,CN)神经元出现的变化及可能涉及的环节。采取腹腔内给药 ,1月后取出用药组、对照组动物耳蜗 ,观察毛细胞 ,绘制耳蜗图 ,对耳蜗核以冠位连续切片 (10 μm) ,利用原位杂交的方法 ,以编码微管蛋白的cDNA片段为探针 ,进行检测。发现与耳蜗图所示全长内毛细胞破坏对应 ,伴随耳蜗核神经元体积缩小 ,耳蜗核内表达微管蛋白的细胞数减少。提示随着耳蜗内毛细胞的损伤、破坏 ,耳蜗核的传入神经冲动刺激被去除 ,影响了耳蜗核团细胞的信号系统的某些环节 ,从而导致细胞体积减少、细胞功能损伤等一系列变化。
To study molecular changes of chinchilla cochlear nucleus (CN) neurons after deprivation of afferent input from cochlear inner hair cells (IHC) which were destroyed by administration of carboplatin. One month after the usage of carboplatin, the normal group, carboplatin treated group were examined to get cochleograms, brain stems were serially sectioned with thickness of 10 μm. The sections were studied for the expression of cytoskeletal mRNA by in situ hybridization method. Cochleograms showed that the IHCs, which closely relate to cochlear nucleus, were getting lost through the entire length of cochlear. The total levels of expression of cytoskeletal mRNA in CN was lower than that of normal at the time examined. So after deprivation of afferent input, the neurons in CN were negatively affected. The changes of cytoskeletal system and the signal transduction system associated with it may be responsible.
出处
《基础医学与临床》
CSCD
北大核心
2004年第3期282-286,共5页
Basic and Clinical Medicine
基金
高等学校博士学科点专项科研基金 (19990 0 2 311)
