摘要
观察血管紧张素Ⅱ受体拮抗剂缬沙坦对实验性糖尿病大鼠肾脏皮质葡萄糖转运蛋白 1(GLUT1 )和转化生长因子 β1 (TGFβ1 )mRNA表达的影响 ,以探讨其保护肾功能的机理。用链尿佐菌素腹腔注射法复制糖尿病大鼠模型 ,动物随机分为糖尿病组、缬沙坦治疗组及对照组 ;分别于实验第 4、6周末测定大鼠平均动脉压、血糖、血肌酐、尿肌酐、肾重 体重、尿白蛋白排泄率及平均肾小球面积和平均肾小球体积。用逆转录 PCR(RT PCR)法对肾皮质GLUT1及TGFβ1 mRNA表达进行半定量分析。在第 4周及第 6周末 ,各组大鼠平均动脉压无差异 ,治疗组肌酐清除率、肾重 体重、尿白蛋白排泄率及肾小球平均面积和平均体积均显著低于同时期的糖尿病组。糖尿病组GLUT1 及TGFβ1mRNA表达较正常组显著增高 ,治疗组二者表达均有所下降。缬沙坦能够下调糖尿病大鼠肾皮质GLUT1 及TGFβ1mRNA表达 ,具有保护肾脏的作用。
To investigate the mechanism of Valsartan in protecting the kidney of streptozotocin-induced diabetic rats Sprague-Dawley rats were randomly divided into diabetic model treated with valsartan[10mg/(kg·d)] group(A), non-treated diabetic model group(B) and normal control group(C). Plasma glucose,kidney mass/body mass ratio, serum creatinine, urinary creatinine and urinary albumin excretion rates were measured in the fourth and sixth week respectively. The expression of GLUT 1 and TGFβ 1 mRNA in renal cortical were measured with RT-PCR in the sixth week rats. Mean glomerular transverse sectional area and mean glomerular volume were calculated by analysis system of the image. In either two stages, kidney mass/body mass ratio,Ccr, 24 urinary albumin excretion rate in both diabetic group (B) were higher than that of normal group(C)(P<0.01),but significantly decreased in group A than that in group B. Mean glomerular transverse sectional area and mean glomerular volume in A were significantly smaller than that of group B(P<0.01). By the end of the sixth week,the expression of GLUT 1 and TGFβ 1mRNA of group B was significantly increased than that of group C in rat kidney cortex(P<0.01), at the same time the expression of group A was decreased than that of group B(P<0.05). The results showed that Valsartan could downregulat the expression of GLUT 1and TGFβ 1mRNA. It could also reduce the level of increasing rate of renal and urinary albumin and so was able to prevent the glomerular sclerosis.
出处
《基础医学与临床》
CSCD
北大核心
2004年第3期287-290,共4页
Basic and Clinical Medicine
基金
河北省自然科学基金 (30 0 36 2 )
