大肠埃希菌的耐药特性及其产超广谱β-内酰胺酶的分析

Antibiotic resistance of Escheria Coli and the profileof extended-spectrum β-lactamases production

观察大肠埃希菌的耐药特性及产超广谱β 内酰胺酶 (ESBLs)的情况 ,为临床正确选择抗生素提供药敏依据。用微量稀释法测定最低抑菌浓度 (MIC) ,用ESBLs表型确证试验检测出产ESBLs的大肠埃希菌。共检出 314株大肠埃希菌 ,对其中 2 98株大肠埃希菌进行了ESBLs检验 ,检出产ESBLs菌 81株 ,检出率为 2 7 2 % ;81株产ESBLs菌对多种药物耐药率较高 ,除亚胺培南、头孢哌酮 舒巴坦、庆大霉素和阿米卡星外 ,大肠埃希菌产ESBLs株对其他 9种抗生素的耐药性明显高于非产ESBLs株。大肠埃希菌产ESBLs率高 ,应引起临床高度重视 ,对检测出的大肠埃希菌均应进行ES BLs检测 ,产ESBLs细菌有较高的交叉耐药性和多重耐药性 ,对亚胺培南和头孢哌酮 舒巴坦的敏感性好。

To investigate extended-spectrum β-lactamase(ESBLs) production by Escheria coli and the analysis of its antibiotic resistance. Dilution test was used to detect minimal inhibitory concentration(MIC). Phenoty confirmatory test was used to detect ESBLs of E.coli RESULT Up To 27.2% of strains were considered as ESBLs in 298 Strains of E coli by phenoty confirmatory test. In addition to imipenem, cefoperazone/sulbactam, amikacin and gentamicin ,the resistant rates of ESBLs-producing strains to other 9 antibiotics were much higher than that of non-ESBLs-Producing strains(P<0.01). The prevalence of ESBLs was high in E. coli,the resistant rates of ESBLs-producing strains to most antibiotics were higher than non- ESBLs-Producing strains. Imipenem, cefoperazone/sulbactam are effective antibiotics to infections caused by ESBLs-producing strains.