HPLC法同时测定6种苯二氮(?)类药物血药浓度

Simultaneous determination of six benzodiazepines drugs in serum by HPLC

目的:建立HPLC法同时测定血清中6种苯二氮(?)类药物(地西泮、硝西泮、氯硝西泮、三唑仑、艾司唑仑、阿普唑仑)浓度。方法:用固相萃取技术处理含药血清,色谱柱为Nova-pak C18柱(150mm×3.9 mm,4 μm);流动相为0.01 mol·L-1磷酸盐缓冲液(pH 2.15)-乙腈(71:29,V/V);检测波长223 nm;流速1 mL·min-1,柱温30℃。结果:6组分经固相萃取提取完全,杂质去除彻底。在该色谱条件下6种药物分离良好,在0.01-10 mg·L-1范围内浓度与峰面积呈良好的线性关系(r>0.9990),相对回收率96％-104％,日内、日间RSD均<9％。结论:该法快速、简便、准确,适用于苯二氮(?)类药物中毒患者的快速鉴定,提高急诊救治水平和指导癫癎治疗用药。

AIM: To establish a rapid and simple analysis method for the determination of 6 benzodiazepines drugs (diazepam, nitrazepam, clonazepam, triazolam, estazolam, alprazolam) in serum simultaneously by HPLC with solid phase extraction. METHODS: Drugs in serum were extracted with accubond SPE C18 column. The analytical column was Nova-pak C18 column (150 mm×3.9 mm,4 μm). The mobile phase consisted of 0.01 mol·L-1 phosphate buffer solution (pH 2.15)-acetonitrile(71:29, V/V). The detection wavelength was 223 nm, the flow rate was 1 mL·min-1 and the column temperature was 30℃. RESULTS: Six drugs were extracted completely, impurities were removed thoroughy. Six drugs were separated well under the chromatographic condition. Every linear range of the six drugs was 0.01-10 mg·L-1 and all correlation coefficient was more than 0.999 0. The relative recoveries of the 6 drugs was 96%-104% respectively. The relative standard deviation(RSD) of within day and between day were all less than 9% . CONCLUSION: This method is found to be rapid, simple,accurate, sensitive and more suitable for identifying the benzodiazepines drugs toxicosis and therapeutic drug monitoring application.