HPLC测定人血浆中苯巴比妥、苯妥英、卡马西平浓度

Simultaneous determination of phenobarbital, phenytoin and carbamazepine in human plasma by HPLC

目的 建立测定人血浆中苯巴比妥、苯妥英、卡马西平浓度的高效液相色谱方法。方法 血浆样品用二氯甲烷萃取。采用Shim-pack CLC-CN色谱柱,以正己皖-无水乙醇-甲醇(94.5:5:0.5)为流动相,紫外检测波长为220 nm,以地西泮为内标。结果 苯巴比妥、苯妥英、卡马西平的线性范围均为0.5-80.0 μg·ml-1(r≥0.9994),方法回收率分别为100.69％,99.66％及101.01％。日内和日间RSD均不大于5.9％。结论 本方法灵敏、特异性强、准确、简便易行,适用于苯巴比妥、苯妥英、卡马西平的治疗药物监测。

OBJECTIVE: To establish a HPLC method for the determination of phenobarbital, phenytoin and carbamazepine in human plasma. METHODS: The plasma samples were extracted by dichloromethane. The separation was performed on a Shim-pack CLC-CN column. The mobile phase was composed of n-hexane-absolute ethanol-methanol (94.5:5:0.5). The detection wavelength was at 220 nm. Diazepam was used as the internal standard. RESULTS: The linearity was obtained over the range of 0.5 ∼ 80.0 μg &middot mL-1 (r ≥ 0.9994) for phenobarbital, phenytoin and carbamazepine with the average recoveries 100.69%, 99.66% and 101.01% respectively. Intra-day RSD and inter-day RSD were less than 5.9%. CONCLUSION: The method appeared to be sensitive, specific, accurate and convenient. It is suitable for the determination of phenobarbital, phenytoin and carbamazepine concentrations in human plasma.