TM和ICAM-1在糖尿病大鼠肾皮质的基因表达

The mRNA Expression of TM and ICAM in Renal Cortex of Diabetic Rat

目的 :用RT -PCR的方法半定量测定STZ诱导的糖尿病大鼠肾皮质中TM及ICAM - 1基因的mRNA表达量 ,以探讨其在糖尿病肾病的发生和发展中可能参与的机制。方法 :选择 18只成年SD大鼠 ,注射STZ成糖尿病模型 ,其中 4只注射胰岛素作为治疗对照组 (TC组 ) ,其余为糖尿病组 (DM组 )。另选 4只作为正常对照组 (NC组 ) ,每 4周测定尿白蛋白定量 ,14周后取肾皮质匀浆后采用RT -PCR检测其中TM和ICAM - 1的mRNA表达 ,并利用半定量法测定其含量变化。结果 :DM组肾皮质内TM和ICAM - 1mRNA的含量与NC组比较有显著差异 ,与TC组无差异 ;DM组TM和ICAM - 1的含量与尿白蛋白定量存在相关性。结论 :糖尿病大鼠的肾皮质内TM和ICAM - 1的mRNA表达相较正常对照组显著上调。且与尿白蛋白排泄存在相关性。提示TM和ICAM - 1可能参与了糖尿病肾病的发生与发展。

Objective: We estimate the mRNA of TM and ICAM-1 in the kidney of the streptozotocin induced diabetic rat, in order to investigate the role of above two in diabetic nephropathy. Methods: 18 of 22 male Sprague-Dawley rats were injected streptozotocin to induce to diabetes(group DM), 4 of them were injected insulin to make as the therapeutic control group(group TC), and the other 4 are the control group(group NC). The content of urine alburmin were assayed every four weeks. Then after 14weeks, we use RT-PCR to detect the expression of TM and ICAM-1 mRNA in the renal cortex of the rats. Results: The TM and ICAM-1 mRNA were increased significantly in kidney of group DM vs group NC (P<0.05), but they were not decreased in the group TC. And the content of urine alburmin was related with the increasement of TM and ICAM-1.Conclusion:TM and ICAM-1 are upregulated in diabetic kidney, they maybe play an important role in the onset and progression of the diabetic nephropathy.