常见舌苔舌上皮细胞凋亡与bax、bc1-2基因表达关系的研究

STUDY ON RELATIONSHIP BETWEEN GENE EXPRESSION OF BAX、BCL-2 AND CELL APOPTOSIS IN TONGUE MYCODERMA EPITHELIUM OF COMMON TONGUE FUR

目的 :研究舌苔厚度变化与舌上皮细胞凋亡、bax、bc1- 2mRNA和蛋白质表达的关系。方法 :运用TUNEL(末端脱氧核苷酸转移酶介导的d -UTP缺口末端标记 )技术、原位杂交、免疫组化和图像分析技术。结果 :与正常薄苔比较 ,剥苔bax基因过度表达伴随细胞凋亡增多 ,而厚苔bax基因低表达伴随细胞凋亡减少。bax基因表达水平变化趋势与细胞凋亡水平变化趋势一致。在各组舌苔中均未检测到bc1- 2基因表达。结论

Objective:To investigate the relationship between the gene expression of baxbc1-2 and cell apoptosis in tongue mycoderma epithelium of common tongue fur.Methods:TUNEL(terminal deoxynucleotidyl transferase-mediated d-UTP nick-end labeling)technique,in stitu hybridization,immunohistochemical technique and image analysis were used in the study. Results:In comparison with normal tongue fur,there was an over-expression of bax in peeling fur and accompanied with up-regulation of apoptosis,while there was an lower-expression of bax in thick tongue fur and accompanied with down-regulation of apoptosis.The change of bax gene expression level had the same tendency with that of cell apoptosis level.The staining of bc1-2 by in situ hybridization and immunohistochemical was negative.Conclusion:The findings suggested that the change of expression level of bax effect cell apoptosis in tongue mycoderma epithelium,thus be one possible cause for the thickness's change of tongue coating.