摘要
目的 :研究舌苔厚度变化与舌上皮细胞凋亡、bax、bc1- 2mRNA和蛋白质表达的关系。方法 :运用TUNEL(末端脱氧核苷酸转移酶介导的d -UTP缺口末端标记 )技术、原位杂交、免疫组化和图像分析技术。结果 :与正常薄苔比较 ,剥苔bax基因过度表达伴随细胞凋亡增多 ,而厚苔bax基因低表达伴随细胞凋亡减少。bax基因表达水平变化趋势与细胞凋亡水平变化趋势一致。在各组舌苔中均未检测到bc1- 2基因表达。结论
Objective:To investigate the relationship between the gene expression of baxbc1-2 and cell apoptosis in tongue mycoderma epithelium of common tongue fur.Methods:TUNEL(terminal deoxynucleotidyl transferase-mediated d-UTP nick-end labeling)technique,in stitu hybridization,immunohistochemical technique and image analysis were used in the study. Results:In comparison with normal tongue fur,there was an over-expression of bax in peeling fur and accompanied with up-regulation of apoptosis,while there was an lower-expression of bax in thick tongue fur and accompanied with down-regulation of apoptosis.The change of bax gene expression level had the same tendency with that of cell apoptosis level.The staining of bc1-2 by in situ hybridization and immunohistochemical was negative.Conclusion:The findings suggested that the change of expression level of bax effect cell apoptosis in tongue mycoderma epithelium,thus be one possible cause for the thickness's change of tongue coating.
出处
《中国中医药科技》
CAS
2004年第4期193-195,共3页
Chinese Journal of Traditional Medical Science and Technology
基金
国家自然科学基金资助项目No.3 9770 890