IL-10、IL-8在慢性前列腺炎中的改变及意义

The Effects of Interleukin-10 and -8 in Chronic Prostatitis

目的 :研究白细胞介素 10 (IL 10 )和IL 8在慢性前列腺炎 (CP)发病机制和诊断中的作用。 方法 :随机选择各种类型的CP患者 2 9例 ,通过详细询问前列腺炎相关病史和临床症状、直肠指检前列腺及前列腺按摩液 (EPS)分析、及对部分患者进行按摩前列腺前后的尿液培养 (PPMT)法来诊断CP。选择 11例生殖功能正常的健康男子作对照。双抗体夹心ELISA法定量分析按摩前列腺后获取的精确控制的前段尿液 (VB3)内的IL 10和IL 8水平。 结果 :具有临床症状的 8例CP患者VB3中IL 10水平 [(4 7.1± 4 .5 )pg/ml]明显高于 11例健康对照者 [(4 0 .8± 5 .7)pg/ml]和 2 1例不育症中的Ⅳ型前列腺炎者 [(4 2 .7± 6 .7)pg/ml],P <0 .0 5 ;具有临床症状的 8例CP患者VB3中IL 8水平 [(1386 .2± 85 2 .6 )pg/ml]和不育症中的 13例Ⅳ型CP患者IL 8水平 [(12 0 3.8± 80 7.8)pg/ml]明显高于 7例健康对照者 [(4 12 .1± 2 17.2 )pg/ml],P <0 .0 5。 结论 :IL 10和IL 8在CP发病机制和诊断中具有重要意义 ,并可以用VB3来代替EPS或精液进行检测。

Objective: To investigate the effects of interleukin-10 (IL-10) and IL-8 in the pathogenesis and diagnosis of chronic prostatitis(CP). Methods: Twenty-nine different types of CP patients with complaints of low urinary duct symptom were selected at random,and the diagnosis of CP was established by inquiry of the history of prostatic disease,digital rectal examination of the prostate,and analysis of express prostatic secretions (EPS) for leukocytes. Bacterial infection of the prostate was detected by pre- and post-message test (PPMT) in some of the inflammatory CP patients. Quantitative evaluation of IL-10 and IL-8 levels in the limited voided bladder 3 (VB3) was performed by ELISA methods. Eleven healthy men without CP were chosen as control. Results: The level of IL-10 [( 47.1 ± 4.5 ) pg/ml] in VB3 in 8 patients with apparent clinical symptoms was significantly higher than in the 11 infertile males[( 40.8 ± 5.7 ) pg/ml] and the 21 patients with both infertility and asymptomatory inflammatory prostatitis(CP type Ⅳ) [( 42.7 ± 6.7 ) pg/ml],P < 0.05 . The levels of IL-8 in the 8 patients with apparent clinical symptoms [( 1 386.2 ± 852.6 ) pg/ml] and in the 13 patients with both infertility and asymptomatory inflammatory prostatitis (CP type Ⅳ) [( 1 203.8 ± 807.8 ) pg/ml] were significantly higher than in the 7 healthy males[ ( 412.1 ± 217.2 ) pg/ml], P < 0.05 . Conclusion: IL-10 and IL-8 had a very important role in etiology of CP,and could be detected in VB3 as well as in routine semen and EPS.