睾丸生精障碍患者血清抗FSH抗体的测定及临床意义

Detection and Potential Function of Anti-FSH Antibodies in Patients with Spermatogenesis Dysfunction

目的 :建立人血清抗卵泡刺激素 (FSH)抗体的酶联免疫吸附测定法 (ELISA) ,探讨血清抗FSH抗体与男性不育的关系。 方法 :用本室建立的FSH抗体ELISA法 ,分别检测 15 0例男性不育患者和 5 0例正常生育男性血清抗FSH抗体水平。 结果 :少弱精子症和无精子症患者抗FSH抗体阳性率分别为 2 2 .4 % (2 2 / 98)和 5 4 .5 % (12 / 2 2 ) ,精子密度、活率正常的男性不育患者抗FSH抗体阳性率为 6 .7% (2 / 30 ) ,生育男性的阳性率则为 4 % (2 / 5 0 )。少、弱精子症患者抗FSH抗体阳性率明显高于精子密度、活率正常的男性不育患者及正常生育男性 (P <0 .0 5 ) ,明显低于无精子症患者 (P <0 .0 5 ) ;精子密度、活率正常的男性不育患者抗FSH抗体阳性率与正常生育男性相比差异无显著性 (P >0 .0 5 )。 结论 :少、弱精子症患者 ,尤其是无精子症患者 ,血清中存在较高水平的抗FSH抗体 ,抗FSH抗体的存在可能与FSH形成抗原抗体复合物 ,进而影响FSH活性 ,妨碍精子发生。

Objective: To detect the anti-FSH antibody using ELISA,and further probe into the role of anti-FSH in infertile patients.Methods: The anti-FSH antibody was detected using ELISA in the serum of patients with spermatogenesis dysfunction,of infertile patients with normal sperm density and motility,and of normal fertile males. Results: The positive rate of anti-FSH antibody in the patients with oligospermia and/or asthenospermia [ 22.4 %(22/98)]was significantly higher than that in the normal fertile ( P < 0.05 )and that in the infertile patients with normal sperm density and motility [ 6.7 %(2/30)]( P < 0.05 ). The positive rate of anti-FSH antibody in the patients with oligospermia and/or asthenospermia was lower than that in the patients with azoospermia [ 54.5 %(12/22)]( P < 0.05 ). There was no significant difference in the positive rate between the normal control and the sterile males with normal sperm density and motility. Conclusion: The anti-FSH antibody may be an important factor to cause spermatogenesis dysfunction by combining FSH to form immune compound and depress the activation of FSH.