降脂颗粒防治脂肪肝的实验研究

Experimental study of Jiangzhikeli on prevention and treatment of fatty liver

目的 观察降脂颗粒对大鼠脂肪肝的预防和治疗作用。方法 标准SD大鼠给予高脂饲料、乙醇灌胃、CCl4菜籽油溶液注射 ,联合建立复合脂肪肝动物模型[1] 。随机分为 10组 ,A1,A2 为空白对照组 ,B1,B2 为模型对照组 ,C ,D为降脂颗粒大、小剂量预防组 ,E为东宝肝泰预防组 ,F、G为降脂颗粒大、小剂量治疗组 ,H为东宝肝泰治疗组 ,各组给予相应药物灌胃处理 ,A1,B1,C ,D ,E组于实验第 16天 ,A2 ,B2 ,F ,G ,H组于实验第 4 6天处死动物 ,取血及肝组织做肝功能、血脂、肝脏内脂质及病理组织学检查。结果 ①C ,D组与B1组比较 ,F ,G组与B2 组比较 ,各项肝功、血脂指标及病理检查结果均有改善 (P均 <0 .0 1)。②C ,D组与E组比较 ,F ,G组与H组比较 ,各项肝功、血脂指标及病理检查结果均无显著性差异 (P均 >0 .0 5 )。结论 降脂颗粒可预防和治疗脂肪肝 ,尤以高剂量预防及治疗组作用显著。

Objective It is to observe the preventing and treating action of Jiangzhikeli on fatty liver.Methods The compound fatty liver models were induced by high-fat feed, pouring the alcohol into stomach and injecting with CCl 4 rapeseed oil, and then were randomly divided into ten groups: blank contrast group (A 1, A 2), model contrast group (B 1, B 2), Jiangzhikeli high, low dose preventive group (C, D), Dongbaogantai preventive group (E), Jiangzhikeli high, low dose therapeutic group (F, G), Dongbaogantai therapeutic group (H). Each group was poured the drugs into stomach. The rats in A 1, B 1, C, D, E groups were killed respectively on the sixteenth day. The rats in A 2, B 2, F, G, H groups were killed respectively on the forty-sixth day. Their blood and liver tissue were taken. Then the liver function, blood fat and lipid in liver were examined and the change of liver histology was observed.Results ①Group C and group D were compared with group B1 and group F and group G were compared with group B2, then all of the liver function, blood fat, lipid in liver and the change of liver histology were got better (P<0.05). ② Group C and group D were compared with group E and group F and group G were compared with group H, then there was not markedly different among the liver function, blood fat, lipid in liver and the change of liver histology (P>0.05).Conclusion Jiangzhikeli can prevent and theat fatty liver, especially the action of high dose preventive and therapeutic groups is more markedly.