“双击”大鼠脾脏IκBα和TLR4基因表达及参麦注射液对其影响

The anti-shock effect of shenmai and the influence on TLR4 and IκBαmRNA expression in "Two-Hit" rat spleen

目的通过观察“双击”(失血性休克+内毒素)大鼠脾脏中IκBα和TLR4mRNA表达及参麦注射液对其的影响,探讨参麦注射液抗休克的分子机制。方法大鼠随机分为单纯失血性休克(HS)组、“双击”(HS+LPS)组、地塞米松(HS+LPS+DEX)组和参麦注射液(HS+LPS+SM)组。大鼠第一次打击为失血性休克(MBP维持在5.3kPa),第二次打击舌下静脉LPS注射(1.5mg/kg),舌下静脉给予参麦注射液(28.8mg/kg)、地塞米松(2mg/kg)。4h后测定脾脏组织中IκBα和TLR4mRNA表达。结果HS+LPS+DEX组和HS+LPS+SM组TLR4mRNA表达高于HS、HS+LPS组;HS+LPS+Dex组和HS+LPS+SM组IκBαmRNA表达低于HS、HS+LPS组。结论参麦注射液能够下调脾脏组织中TLR4mRNA表达,同时上调脾脏组织中IκBα的表达,提示参麦可能通过调节NF-κB信号转导途径抑制炎症反应来对机体细胞起保护作用。

Objective To explore the molecular mechanism of the anti-shock effect of Shenmai and observe the influence on TLR4 and IκBαmRNA expression in 'Two-Hit' Rat Spleen. Method Rats were divided into HS, HS+LPS,HS+LPS+DEX, HS+LPS+SM group respectively. TLR4mRNA and IκBαm RNA expression were assayed 4h after treated by 'two-hit'. Results Compared with the HS or HS+LPS group, TLR4mRNA expression in the experimental groups were increased, of which HS+LPS+DEX and HS+LPS+SM group were lower obviously than HS and HS+LPS group. IκBαmRNA expression in the experimental groups were decreased, of which HS+LPS+DEX and HS+LPS+SM group were higher obviously than HS and HS+LPS group. Conclusion SM may down-regulate TLR4mRNA expression and up-regulate IκBαmRNA expression in the spleen tissue. SM has a protective effect on the body tissue injury from hemorrhagic shock and endotoxic shock.