G蛋白β_3亚单位基因C825T多态性与高血压患者左室肥厚的相关性

Correlation between G protein β_3 subunit gene polymorphism and left ventricular hypertrophy in hpertensive patients

目的 探讨G蛋白 β3 亚单位基因C82 5T多态性与中国人原发性高血压 (EH )及左室肥厚的关系。方法 采用多聚酶链式反应结合限制性内切酶片段长度多态分析方法检测 14 7例健康人和 3 2 1例高血压患者的G蛋白 β3 亚单位C82 5T多态性及用超声测定高血压患者的左室重量 (LVM )、左室重量指数 (LVMI)、室间隔厚度 (IVST)。结果 高血压组G蛋白 β3 亚单位C82 5T多态性中基因型频率 (CC 2 8.7%、CT 5 2 %、TT 19.3 % )、等位基因频率 (C 5 4.672 %、T 45 .3 3 % )与正常对照组基因型频率 (CC 2 7.2 %、CT 46.9%、TT 2 5 .9% )、等位基因频率 (C 5 0 .7%、T 49.3 % )比较无显著性差异 (P >0 .0 5 ) ;CT+TT基因型携带者LVM (2 65 .2 5± 5 4.3 4g)、LVMI(14 4.42± 2 4.73g/m2 )、IVST(1.169± 0 .16cm )均高于CC基因型携带者(2 3 8.81± 3 9.49g、13 4.3 6± 18.3 3g/m2 、1.10 14 7± 0 .14cm) ,有统计学差异 (P <0 .0 5 )。结论 G蛋白 β3 亚单位基因C82 5T多态性可能与中国人原发性高血压发病无关 ,82

Objective To investigate the relationshiop between G protein β 3 subunit gene polymorphism and essential hypertension and left ventricular hypertrophy in a chinese population. Methods Polymerase chain reaction combined with restriction enzyme digestion was used to detect the polymorphism of G protein of β 3 subunit gene in 147 normotensive controls and 321 hypertensive patients. Left ventricular mass (LVM), left ventricular mass index (LVMI), intraventricular septem thicks (IVST) were measured by echocardiography in hypertensive patients. Results There were no significant differences of the GNB3 gene C825T polymorphism genotypes and alleles between hypertensive patients (CC 28.7%, CT 52%, TT 19.3% , C 50.7%, T 49.3%) and normotensive controls (CC 27.2%, CT 46.9%, TT 25.9%, C 50.7%, T 49.3%). LVM (265.25±54.34 g), LVMI (144.42±24.73 g/m 2), IVST (1.169±0.16 cm)of CT + TT genotype were higher than CC genotype (238.81±39.49 g, 134.36±18.33 g/m 2, 1.10147±0.14 cm) in hypertensive patients. Conclusion The C825T polymorphism of G protein β 3 subunit was not associated with EH in Chinese. 825T allele of GNB3 gene probobly was a genetic risk factor of left ventricular hypertrophy in hypertensive patients.