幽门螺旋杆菌诱导基质金属蛋白酶-9表达的研究

The Induction of Matrix Metalloproteinase-9 Expression by Helicobacterium Pylori

目的 通过研究幽门螺旋杆菌对基质金属蛋白酶表达水平的影响 ,探讨幽门螺旋杆菌致病的分子机理。方法 ATCC495 0 3 (CagA+ )、HP0 3 0 92 9(CagA-)幽门螺旋杆菌分别与BGC -82 3共同培养后提取总RNA ,用RT -PCR方法分析BGC -82 3细胞中MMP -9的表达水平 ;通过胃镜活检选取 9例HP阳性胃溃疡、9例HP阴性胃溃疡患者的标本采用RT -PCR分析MMP -9的表达水平 ;取 17例HP阳性和 14例HP阴性的胃溃疡标本经切片后石蜡包埋 ,用免疫组化染色检测MMP -9表达并图象定量分析测量MMP -9染色阳性的面积。结果 HP能诱导MMP -9在BGC -82 3细胞中表达 ,CagA+ HP株明显强于CagA-株 ;在HP阳性胃溃疡的胃黏膜上皮细胞中 ,MMP -9表达水平明显高于HP阴性胃溃疡。结论 HP诱导MMP -9的表达在胃溃疡。

Objective To study the pathogenic molecular mechanism of H.pylori through analyzing its effect of on MMP-9 expression. Methods BGC-823 cells were cocultured with ATCC49503(CagA +)and HP 030811 (CagA -) H.pylori strains respectively, then the total cellelar RNA was extracted. The MMP-9 mRNA expression in BGC-823 cells, 9 cases of H.pylori-positive and 9 cases of H.pylori-negative gastric ulcer biopsy tissues was detected by RT-PCR. The MMP-9 protein expression on the paraffin-imbedded tissue sections of 17 cases of H.pylori-positive and 14 cases of H.pylori-negative gastric ulcer tissues was detected with immunohistochemical technique, and was quantified by image quantitative analysis. Results H.pylori induced MMP-9 expression in BGC-823, and the level of MMP-9 expression induced by CagA -postive H.pylori strain was higher than that by CagA -negative one. MMP-9 expression level was higher in the H.pylori-positive gastric ulcer tissues than that in the H.pylori-negative ones. Conclusion H.pylori-induced MMP-9 expression may play an important role in gastric ulcer formation and gastric carcinogenesis.