颅外垂体移植术供体细胞的培养研究

Culture of Donor Cells in Extracranial Pituitary Implantation

目的 本实验通过对人垂体腺瘤细胞体外培养实验 ,证实培养技术的可靠性和可重复性 ;为颅外移植术应用垂体腺瘤细胞作为移植物供体细胞的实施 ,以及为腺垂体移植细胞库的建立提供有用的实验依据。方法 利用手术切除的人垂体腺瘤组织做体外培养 ,动态观察培养细胞的生长状态 ,通过放免测定和免疫化学染色技术 ,评价体外培养的垂体细胞的功能状态。结果 人垂体腺瘤细胞经体外培养仍具有分泌激素的功能 ,6例GH腺瘤均有大量GH分泌 ,5例PRL腺瘤分泌大量PRL ,3例无功能腺瘤细胞培养液中分别有不同程度的FSH及LH、PRL分泌。结论 培养成功的 14例人垂体腺瘤细胞在体外均具有激素分泌功能。为应用垂体腺瘤细胞为移植物供体 。

Objective To culture human pituitary adenoma cells to provide some useful experimental foundations to practice pituitary adenoma cells as donor cells in the extracranial implantation. Methods Human pituitary adenoma cells obtained by operation were cultured in vitro, and their growth state was dynamically observed. Immunohistochemical staining and RIA were used to evaluate their functional status. Results The cultured pituitary adenoma cells still had secretory function in vitro. 6 cases of GH adenoma cells secreted a large amount of GH, 5 cases of PRL adenoma cells secreted a large amount of PRL, and 3 cases of pituitary adenoma cells without function could also secrete a small quantity of FSH, LH and PRL. Conclusion 14 cases of cultured human pituitary adenoma cells had secretory function in vitro, which provides the useful experimental data to perform extracranial implantation with pituitary adenoma cells as donor cells.