海洛因成瘾大鼠心电图及心肌超微结构改变的研究

tudy on the changes of electrocardiogram and ultrastructural in heroin dependence in rats

目的探讨海洛因成瘾大鼠心电图及心肌超微结构的改变,为海洛因对心脏的损害机制提供研究基础。方法建立大鼠海洛因成瘾模型,观察心电图、HE染色及心肌超微结构改变。结果大鼠海洛因成瘾组心电图改变明显,主要表现在心率减慢、P波及T波压低、时间延长,S-T段压低、时间延长,QT间期延长,上述差异有统计学意义(P<0.05),提示心肌损伤、心肌缺血及心室功能下降。电镜改变主要表现在核浓缩,核变小,核膜皱缩,染色质凝集成块,线粒体嵴排列紊乱、消失及空泡变等,提示海洛因可造成心肌细胞超微结构的病理改变。结论海洛因对心肌可造成损害,并且心肌超微结构改变提示心肌凋亡可能是海洛因造成心肌损伤的机制之一。

Objective To study the changes of electrocardiograms(ECG)and myocardial ultrastructure in heroin dependence in rats,in order to reveal the mechanisms of the myocardial injury by heroin.Meth-ods Establish heroin addict model in SD mice,investigate the changes in electrocardiograms,HE staining and myocardial ultrastructure.Results The electrocardiograms of the addict group had prominently changes,main expressions:heart rate decreased,P wave and T wave amplitude reduced and duration in-creased,S-T reduced and duration increased,QT interval prolongation,these changes indicated that my-ocardium had been injured,myocardial ischemia,ventricle function declined.These difference was signifi-cant(P<0.05)between before inject heroin and after inject heroin.Transformations in the ultrastructure:nuclear concentrate,reduce,nuclear membrane shrink,chromatin agglutinate,mitochondria cristal had dis-order formation,disappeared or hollowed,these indicated that heroin could cause pathological changes in myocardial ultrastructure.Conclusion Above-mentioned changes indicated that heroin can injure myocardi-um,and the changes of myocardial ultrastructure suggested that myocardial apoptosis may be one of the mechanisms of the myocardial injury by heroin.