人类白细胞抗原-B51与Behet病葡萄膜炎的相关性研究

Association of human leukocyte angtigen-B51 and Behet′s disease with uveitis

目的 探讨人类白细胞抗原 (HL A) - B5 1与 Behcet ) 病葡萄膜炎间的相关性。 方法 应用微量淋巴细胞毒试验 ,对 2 7例 Behcet ) 病患者和 30例健康人的 HL A- A和 HL A- B抗原进行研究。应用聚合酶链式反应 -序列特异性引物 (PCR- SSP)技术测定 HL A- B5 1抗原阳性的 Behcet ) 病患者和对照组人群中的HL A- B5 1等位基因 (HL A- B* 5 10 1- B* 5 10 7)。 结果 Behcet ) 病患者和对照组 HL A- B5 1的阳性频率分别为 6 3%和 16 .7% [χ2 =12 .9,P<0 .0 0 1,Pc<0 .0 5 ,相对危险度 (RR) =8.5 ]。在 Behcet ) 病患者中 ,葡萄膜炎的患病率为 4 0 .7% (11/ 2 7) ;72 .7% (8/ 11) Behcet ) 病葡萄膜炎中存在 HL A- B5 1(均为 HL A- B* 5 10 1)。HL A- B5 1和 HL A- B* 5 10 1与 Behcet ) 病葡萄膜炎存在弱相关性 ,但均未见统计学差异 (分别为 RR=2 .0 7,χ2 =0 .75 9,P >0 .2 5 ;RR =2 .6 7,χ2 =1.395 ,P >0 .10 )。 结论 提示 HL A- B5 1(HL A- B* 5 10 1)是Behcet ) 病的易感基因 。

Objective To investigate the relationship between human leukocyte antigen(HLA)-B51 and Behcet′s) disease (BD) with uveities. Methods HLA-A and HLA-B antigen of 27 pateints with BD and 30 healthy persons were detected by microlymphocyte toxicity asssay. HLA-B51 allele (HLA-B*5101-B*5107) in BD patients with positive HLA-B51 antigen and the controls was detected by polymerase chain reaction-sequence specific primer (PCR-SSP). Results The positive rate of HLA-B51 was 63% and 16.7% in BD patients and the controls, respectively (χ2=12.9, P<0.001, Pc<0.05,RR=8.5). Uveities was found in 11 out of 27 BD patients with uveitis. No relativity was found between HLA-B51 and uveitis in BD patients (RR=2.07, χ2=0.759,P>0.25), and weak association was found between HLA-B*5101 and uveitis (RR=2.67, χ2=1.395, P>0.10). Conclusions HLA-B51 might be a susceptible gene for BD, and there was a weak association between HLA-B51(HLA-B*5101) and BD patients with uveitis.