视网膜母细胞瘤内多种凋亡相关蛋白的检测

Detection of multiple apoptosis related proteins in retinoblastoma

目的 探讨 survivin在视网膜母细胞瘤 (RB)中的表达及其与 RB临床分期、组织分化程度及 p5 3、bcl- 2蛋白表达的关系。 方法 应用抗 survivin、p5 3和 bcl- 2单克隆抗体 ,采用链霉菌抗生物素蛋白 -过氧化酶免疫组织化学法对 38例 RB常规石蜡标本行 survivin、p5 3和 bcl- 2蛋白表达的测定 ,对 6例正常视网膜组织行 survivin表达的测定。 结果 38例 RB组织中 2 0例 survivin蛋白呈阳性表达 ,占5 2 .6 % ,6例正常视网膜组织内均无 survivin蛋白表达 ,两组比较差异有显著性的意义 (P<0 .0 5 )。survivin的阳性表达与患者的性别、临床分期、组织学分型无关 (P>0 .0 5 )。 38例 RB组织中 ,2 5例 p5 3阳性表达 ,18例 bcl- 2阳性表达 ,阳性表达率分别为 6 5 .8%和 4 7.4 %。p5 3、bcl- 2阳性表达组中 survivin阳性表达率均显著高于 p5 3、bcl- 2阴性表达组 (P均 <0 .0 5 )。 结论 survivin在 RB中表达上调 ,提示该基因可能在RB的发生发展中起一定作用 ;survivin、p5 3和 bcl- 2可能通过相互作用共同参与 RB的发生发展。

Objective To explore the expression of survivin gene in retinoblastoma (RB) and its relationship with the stages and histodifferentiation degree of RB and the expression of p53?bcl-2 proteins. Methods Expression of survivin, p53 and bcl-2 proteins in 38 RB conventional paraffin samples were detected with survivin, p53 and bcl-2 monoclonal antibodies respectively by immunohistochemical assay. The expression of survivin of normal retina in 6 control samples was observed. Results In 38 cases of RB, positive expression of survivin was found in 20 (52.6%); while none of the 6 normal retinal tissue expressed survivin, which had significant difference between the two group (P<0.05). The positive expression of survivin did not correlate with sex of patient, disease stages and histological type (P>0.05). In 38 RB cases, positive expression of p53 was in 25 with the rate of 65.8%, and of bcl-2 in 18 with the rate of 47.4%. The positive-expressed rates were much higher in positive-expressed p53 and bcl-2 group than those in the negative-expressed p53 and bcl-2 group (P<0.05). Conclusion The increase of the expression of survivin implies that it may take part in the occurrence and development of RB; the interaction among survivin, p53 and bcl-2 may participate in the access and the course of RB.