大鼠视网膜脱离及复位状态下存活的视网膜神经节细胞计数

The numerical count of survivor retinal ganglion cells in detached and reattached retina in adult rats

目的 探讨神经节细胞 (RGC)在实验性视网膜脱离及复位状态下的反应 ,检测白介素 - 1β(IL - 1β)、白介素 - 1受体拮抗物 (IL - 1Ra)在 RGC丢失过程中的作用。 方法 73只 SD大鼠用 1%荧光金(FG)经上丘、外侧膝状体逆行标记 ,healon GV(1.4 %透明质酸钠 )鼻侧视网膜下注射。 10 ng IL - 1Ra和5 0 0 ng IL - 1β抗体随 healon GV一同注入视网膜下。对逆行标记后 10 d,视网膜脱离后 2 h、1、3、5、7、10、2 0、5 0、90 d,视网膜脱离 10 d复位 30 d,视网膜脱离 90 d复位 2 0 d,IL - 1Ra和 IL - 1β抗体视网膜下注入后 1d和 10 d取材的视网膜进行铺片 ,在荧光显微镜下拍片 ,进行 RGC计数。对照组则在球内注入相应计量healon GV。 结果 视网膜脱离后 2 h开始有 RGC的丢失 ,1d时达到高峰 ,然后缓慢下降。在未脱离区 ,亦有 RGC的丢失。视网膜脱离 10 d后复位 (早期复位 )可停止 RGC的丢失 ,视网膜脱离 90 d后复位 (晚期复位 )可使 RGC进一步丢失 ,最后停留在某一水平。IL- 1β抗体、IL- 1Ra可以减少这种丢失。 结论 视网膜脱离状态下 ,脱离区与未脱离区都有 RGC的丢失。早期复位可使 RGC的丢失停止 ,晚期复位可进一步损伤 RGC。拮抗 IL- 1β能减少 RGC的丢失 ,IL- 1Ra对 RGC有保护作用。

Objective To investigate the response of retinal ganglion cells (RGC)in detached and reattached retina in adult rats, and the effect of IL-1beta antibody and IL-1Ra on the loss of RGC. Methods A total of 73 Sprague-Dawley (SD) rats were subretinally injected with healon GV(1.4% hyaluronate)and retrograde labeled with fluorogold (FG), and 10 ng IL-1Ra and 500 ng IL-1beta antibody were injected into the subretinal space combined with healon GV. The retinal flakes were observed under the fluoroscope and the number of RGC was counted 2 hours, 1, 3, 5, 7, 10, 20, 50, and 90 days after detachment; 10 days after detachment and 30 days after reattachement; 90 days after detachment and 20 days after reattachement, and 1 and 10 days after injection with IL-1beta antibody and IL-1Ra,respectively. And the control group was only developed an intraocular injection of the same valume of healon GV. Result Two hours after detachment, the RGC loss was found, reached the peak at first day, and decreased gradually. RGC loss was also found in the non-detached area. The reattachment 10 days after detachment (early reattachment) stopped the loss of RGC, and the reattachment 90 days after detachment (late reattachment) promoted the loss, which rested on a certain level. Subretinal space injection of IL-1Ra and IL-1beta antibody decreased the loss of RGCs in the detached retina. Conclusion The RGCs loss were found both in the detached and attached retina. Early reattachment may stop the loss of RGC, and late reattachment may promote the loss. Both IL-1beta antibody and IL-1Ra have neuroprotective effect on RGC.