HPLC测定妇炎灵泡腾片中大黄素、大黄酚的含量

Determination of emodin and chrysophanol in Fuyanling Effervescent Tablets by HPLC

目的 :建立HPLC法同时测定妇炎灵泡腾片 (大黄、金银花、山豆根等 )中的大黄素、大黄酚的含量测定方法。方法 :采用HPLC法 ,使用C1 8柱 :流动相为甲醇 0 .1 %磷酸混合溶液 (85∶1 5 ) ,检测波长 2 5 4nm。结果 :线性范围 :大黄素 5 0 .2～ 4 0 1 .6ng ,r =0 .9999;大黄酚在 4 9.9～ 399.2ng ,r =0 .9994。样品平均回收率为 :大黄素 99.4 %、RSD =2 .2 ;大黄酚1 0 0 .2 % ,RSD =1 .1 %。结论 :本法简便、快速、结果令人满意 ,可用于妇炎灵泡腾片质量控质方法之一。

AIM: To develop a method to determine the content of emodin and chrysophanol in Fuyanling Effervescent Tablets(Radix et Rhizoma Rhei, Flos Lonicerae, Radix Sophorae Tonkinensis, etc.). METHODS: HPLC was used to determine the content of emodin and chrysophanol in Fuyanling Effervescent Tablets. The separation was performed on YWG ODS column with methanol (0.1%) phosphoric acid(85∶15) mixture as a mobile phase and the wavelength of UV detector was at 254nm. RESULTS: The resolution and the linearity of this method was good. in the range of emodin was 50.2～401.6ng( r =0.9999); chrysophanol was 49.9～ 399.2 ng( r =0.9994); and with the average recovery of emodin: 99.4%( RSD =2.2%); chrysophanol:100%( RSD =1.1%). CONCLUSION: The method is simple, rapid and satisfactory and suitable for quality control of Fuyanling Effervescent Tablets.