六味地黄汤对雄性大鼠生殖系统的影响

Effect of Liuweidihuang Decoction on reproductive system of male rat

目的 :研究六味地黄汤对雄性大鼠生殖系统的作用。方法 :用醋酸棉酚建造大鼠生精障碍模型后 ,用六味地黄汤(分煎与合煎 )ig治疗 ,以市售品成药和甲基睾酮作为对照和阳性对照 ,计数各实验组大鼠的精子数量 ,考察各实验组大鼠精子的质量 ,观察睾丸曲细精管的结构 ,检测血清NO、T、FSH、LH水平。结果 :六味地黄汤治疗组大鼠的精子数量与模型组有显著差异 (P <0 .0 1 ) ,并高于阳性对照组 ;精子活力也显著优于模型组 ;模型组大鼠血清的NO、T均低于六味地黄汤治疗组。结论 :六味地黄汤可显著地促进生精障碍大鼠的精子发生 ,同时可显著提高精子的质量 ;对损伤的睾丸曲细精管有较好的修复作用 ;可提高模型大鼠血清的NO、LH和T水平 。

AIM: To study Liuweidihuang Decoction(LOD) on reproductive system of male rat. METHODS: The model of spermatogenesis obstructive rat was established with gossypol acetate, then Liuweidihuang Decoction was administered to Wistar rat for control group in comparison with “Zhong Jing” LDO Pills positive group and methyltestosterone group. The sperm quantity was taken count of. The sperm quality and the structure of testis seminiferous tubule were observed. The concentration of NO,T,FSH,LH on serum was detected. RESULTS: The sperm quantity of LDD was significantly different from model group. The level of NO and T on serum of model group were lower than LW treated groups. CONCLUSION: LDO could improve the quantity and quality of sperm and NO,T,LH level on serum in rats, and reduce FSH level, repair the traumatic testis seminiferous tubule, and boost the process of spermatogenesis in rats.