摘要
采用毛细管电泳 -迎头分析模式体外实验测定了人血清白蛋白溶液、人血浆、兔血清和血浆样品溶液中游离氯氮平的浓度 .根据前沿峰的峰高直接测定了样品溶液中的游离氯氮平浓度 ,并与传统超滤法进行了比较 .通过考察施加的电压和运行缓冲液的组成对氯氮平电泳峰平台形成的影响确定了最优分离条件 .
Determination of the unbound clozapine concentration in human serum albumin (HSA), human plasma, rabbit serum and plasma samples by capillary electrophoresis in the frontal analysis mode (CE/FA) was developed. The unbound clozapine concentration was directly measured from the height of the frontal peak and the obtained results were compared with those of the conventional ultrafiltration method. Effects of separation voltage and composition of running buffer on the plateau were investigated and the optimized separation condition was determined. The protein binding selectivity of clozapine was discussed.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2004年第13期1256-1259,J004,共5页
Acta Chimica Sinica
基金
国家自然科学基金 (No.2 99750 1 8)资助项目
关键词
血清
血浆
氯氮平
游离浓度
毛细管电泳-迎头分析法
抗精神病药物
clozapine, HPCE/FA (high performance capillary electrophoresis-frontal analysis), protein binding, serum and plasma
