TGF-β_2,p38MAPK在哮喘气道重塑模型中的表达

Expression of TGF-β_2,p38MAPK in a model of asthmatic airway remodeling

目的 :建立哮喘气道重塑模型并检测IL - 13、TGF - β2 在气道重塑中的表达。方法 :建立气道重塑模型 ,光镜、电镜下观察形态学改变 ;应用原位杂交技术检测IL - 13、TGF - β2 mRNA表达 ,Westernblot检测p38MAPK蛋白表达。结果 :气道重塑模型表现为明显的上皮增生 ,粘液产生增加 ,平滑肌细胞增生、上皮下大量胶原沉积 ,同时伴显著的炎症细胞浸润 ,显著表达IL - 13,TGF - β2 mRNA及p38MAPK蛋白。结论 :气道重塑肺组织显著表达IL -13、TGF - β2 mRNA、p38MAPK ,表明Th2细胞因子、TGF - β在气道重塑中可能起一定作用 。

AIM: To detect the expression of IL-13 and TGF-β during airway remodeling. METHODS: A airway remodeling model was established and histological changes were detected under light or electronic microscope. In situ hybridization assays were adopted to determine the status of IL-13 and TGF-β_2 transcript in lung tissue and Western blot was used to detect the expression of p38MAPK protein. RESULTS: Proliferation of airway epithelium and smooth muscle and mucous plugs were found in lung tissue of airway remodeling mice , accompanied by infiltration of eosinophils and lymphocytes in airway. Overexpression of IL-13,TGF-β_2 mRNA and p38MAPK were also observed in airway remodeling group. CONCLUSION: Expression of IL-13, TGF-β_2 mRNA and p38MAPK protein increased significantly during airway remodeling, which may suggest an role for Th2 cytokines, TGF-β_2 in the pathogenesis of airway remodeling. p38 MAPK may be associated with the development of asthma and airway remodeling. [